Department of Evidence-Based Medicine, School of Public Health, Southwest Medical University, 1 Xianglin Road, Luzhou, 646000, Sichuan, People's Republic of China.
Department of Rheumatology and Immunology, Affiliated Hospital of Southwest Medical University, 25 Taiping Road, Luzhou, 646000, Sichuan, People's Republic of China.
Sci Rep. 2021 Jan 8;11(1):10. doi: 10.1038/s41598-020-79544-z.
This study aimed to discuss association between serum Angiopoietin2 (Ang2) levels, Ang2 gene polymorphisms and systemic lupus erythematosus (SLE) susceptibility. It was carried out by 235 SLE, 342 other inflammatory autoimmune diseases patients and 380 healthy individuals. Serum Ang2 levels was examinated by ELISA, and Ang2 rs12674822, rs1823375, rs1868554, rs2442598, rs3739390 and rs734701 polymorphisms were genotyped using KASP. Increased Ang2 concentrations in SLE patients were observed compared with healthy controls and patients with other inflammatory autoimmune diseases. For allelic contrast, except for rs1823375 (P = 0.058) and rs2442598 (P = 0.523), frequencies of alleles for other polymorphisms were significantly different between SLE patients and controls. Genotypes for rs12674822 (TT), rs1868554 (TT, TA and TT+TA), rs734701 (TT) were negatively correlated with SLE susceptibility (OR = 0.564 for rs12674822; OR = 0.572, OR = 0.625, OR = 0.607 for rs1868554; OR = 0.580 for rs734701). Patients carrying rs1868554 T allele and rs3739390 G allele were more likely to develop hematuria (P = 0.039; P = 0.003). The G allele frequencies of rs12674822 and rs2442598 were higher in SLE patients with proteinuria (P = 0.043; P = 0.043). GC genotype frequency of rs3739390 was higher in patients with ds-DNA (+) (P = 0.024). In summary, SLE had increased serum Ang2, which may be a potential biomarker, and the polymorphisms correlated with SLE.
本研究旨在探讨血清血管生成素 2 (Ang2) 水平、Ang2 基因多态性与系统性红斑狼疮 (SLE) 易感性之间的关联。该研究共纳入 235 例 SLE 患者、342 例其他炎症性自身免疫性疾病患者和 380 名健康对照者。采用 ELISA 法检测血清 Ang2 水平，采用 KASP 法检测 Ang2 rs12674822、rs1823375、rs1868554、rs2442598、rs3739390 和 rs734701 多态性。与健康对照者和其他炎症性自身免疫性疾病患者相比，SLE 患者的血清 Ang2 浓度升高。在等位基因对比方面，除 rs1823375（P = 0.058）和 rs2442598（P = 0.523）外，SLE 患者与对照组之间其他多态性的等位基因频率存在显著差异。rs12674822（TT）、rs1868554（TT、TA 和 TT+TA）、rs734701（TT）基因型与 SLE 易感性呈负相关（rs12674822 的 OR 值为 0.564；rs1868554 的 OR 值为 0.572、OR 值为 0.625、OR 值为 0.607；rs734701 的 OR 值为 0.580）。携带 rs1868554 T 等位基因和 rs3739390 G 等位基因的患者更易发生血尿（P = 0.039；P = 0.003）。rs12674822 和 rs2442598 的 G 等位基因频率在蛋白尿患者中较高（P = 0.043；P = 0.043）。rs3739390 的 GC 基因型频率在 ds-DNA(+) 患者中较高（P = 0.024）。综上所述，SLE 患者血清 Ang2 水平升高，可能是一种潜在的生物标志物，且多态性与 SLE 相关。
