Wang Bin, Lv Yunxiao, Ye Shenjian, Zhao Jin, Pan Xinling
Department of Hepatobiliary Surgery and Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, China.
Department of Biomedical Sciences Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, China.
Genet Test Mol Biomarkers. 2023 Jun;27(6):193-198. doi: 10.1089/gtmb.2022.0198.
Angiopoietin-2 Ang2)-mediated angiogenesis plays a crucial role in the pathogenesis of vascular-rich cancers. However, the genetic polymorphism and expression level of Ang2 in patients with primary liver cancer remain unknown. This study included 234 primary liver cancer patients and 199 healthy controls. The expression levels of Ang2 in liver cancer tissues and plasma were determined. Peripheral blood samples were collected to test five single nucleotide polymorphisms (rs2442598, rs734701, rs1823375, rs11137037, and rs12674822). Plasma Ang2 levels in patients with liver cancer were upregulated compared with that in healthy controls. The upregulation of plasma Ang2 levels was significantly associated with vascular invasion, metastasis, and clinical stage. Notably, the transcription level of was elevated in tumor tissues compared with para-carcinoma tissues. Individuals with the TT genotype at rs2442598 and genotype AC and AC+CC at rs11137037 had higher liver cancer risk compared with healthy controls. Upregulated Ang2 levels in blood plasma and cancer tissues of liver cancer patients confirm that Ang2 plays a vital role in the pathogenesis of liver cancer. rs2442588 and rs11137037 are associated with liver cancer risk, thereby highlighting their role in screening individuals susceptible to liver cancer.
血管生成素-2（Ang2）介导的血管生成在富含血管的癌症发病机制中起关键作用。然而，原发性肝癌患者中Ang2的基因多态性和表达水平仍不清楚。本研究纳入了234例原发性肝癌患者和199例健康对照。测定了肝癌组织和血浆中Ang2的表达水平。采集外周血样本检测5个单核苷酸多态性（rs2442598、rs734701、rs1823375、rs11137037和rs12674822）。与健康对照相比，肝癌患者血浆Ang2水平上调。血浆Ang2水平上调与血管侵犯、转移及临床分期显著相关。值得注意的是，与癌旁组织相比，肿瘤组织中Ang2的转录水平升高。rs2442598位点TT基因型以及rs11137037位点AC和AC+CC基因型的个体与健康对照相比患肝癌风险更高。肝癌患者血浆和癌组织中Ang2水平上调证实Ang2在肝癌发病机制中起重要作用。rs2442588和rs11137037与肝癌风险相关，从而突出了它们在筛查肝癌易感个体中的作用。
