College of Food Science, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. 11099, Taif, 21944, Saudi Arabia.
Sci Rep. 2021 Jan 8;11(1):55. doi: 10.1038/s41598-020-80056-z.
Chitosan coating (B/CH) in addition with nano-material films as silicon (B/CH/Nano-SiO) and titanium (B/CH/Nano-TiO) dioxides were developed and applied to detect potential changes on fresh blueberry fruits in commercial storage temperature. Physical, mechanical parameters (weight loss, decay rate, colour index and firmness), phytochemical contents (ascorbic acid, acidity, soluble solids concentration, titratable acidity, and repining index), phenolic enzymes (peroxidase and polyphenoloxidase), pigments (anthocyanin) and microbiological analysis (mesophilic aerobic, yeasts and molds populations) were detected every other day until the end of the experiment. Nano-coating based on (Nano-TiO) established the most suitable values for weight loss (2.22%), titratable acidity (0.45% citric acid), and repining index. (B/CH/Nano-TiO) reported a gradual increase in polyphenoloxidase and peroxidase enzyme activities (659.45 U/min g) and (20.39 U/min g), respectively. While, (B/CH/Nano-SiO) established the slightest change in acidity (2.61), anthocyanin (105.19 cyanidin-3-O-glucoside mg/100 g FW) and minimized the growth of mesophilic aerobic, yeasts, and molds populations (3.73-3.98 log CFU/g), respectively. (B/CH) films maintained lightness (6.80% loss) and recorded the highest ascorbic acid content (7.34 g/100 g FW). Therefore, chitosan nano-material films can maintain nutrients and control the microbial growth for extending the shelf life of fresh blueberry fruits.
壳聚糖涂层（B/CH），外加纳米材料薄膜，如硅（B/CH/Nano-SiO）和钛（B/CH/Nano-TiO）的二氧化物，被开发并应用于检测商业储存温度下新鲜蓝莓果实的潜在变化。物理和机械参数（失重率、腐烂率、色泽指数和硬度）、植物化学物质含量（抗坏血酸、酸度、可溶性固形物浓度、可滴定酸度和回甜指数）、酚酶（过氧化物酶和多酚氧化酶）、色素（花青素）和微生物分析（需氧嗜温菌、酵母和霉菌）每两天检测一次，直到实验结束。基于（Nano-TiO）的纳米涂层为失重率（2.22%）、可滴定酸度（0.45%柠檬酸）和回甜指数建立了最适宜的值。（B/CH/Nano-TiO）报告了多酚氧化酶和过氧化物酶活性（分别为 659.45 U/min g 和 20.39 U/min g）的逐渐增加。而（B/CH/Nano-SiO）在酸度（2.61）、花青素（105.19 矢车菊素-3-O-葡萄糖苷 mg/100 g FW）和需氧嗜温菌、酵母和霉菌的生长（分别为 3.73-3.98 log CFU/g）方面变化最小。（B/CH）薄膜保持了明度（6.80%的损失），并记录了最高的抗坏血酸含量（7.34 g/100 g FW）。因此，壳聚糖纳米材料薄膜可以保持营养物质并控制微生物生长，从而延长新鲜蓝莓果实的货架期。
