Department of Stomatology, The Second Xiangya Hospital, Central South University.
Department of Dermatology, CR and WISCO General Hospital.
Dent Mater J. 2020 Dec 3;39(6):933-940. doi: 10.4012/dmj.2019-255. Epub 2020 Oct 7.
Apigenin is a type of flavonols that exhibits anti-caries properties. Bacterial adherence is the initial step in the forming of a stable biofilm that leads to caries. Bacterial adherence is affected by surface characteristics, including hydrophobicity and bacterial aggregation. However, the effect of apigenin on surface characteristics of cariogenic bacteria has not been reported. We aimed to examine the effects of apigenin on adherence and biofilm formation of Streptococcus mutans UA159. Hydrophobicity and bacterial aggregation, pac and gbpC gene expressions, and cytotoxicity on human dental pulp cells were also determined. Apigenin significantly inhibited the adherence and biofilm formation of S. mutans. Hydrophobicity decreased, whereas the aggregation rate was significantly increased compared with the control. Apigenin significantly suppressed pac and gbpC gene expressions. Apigenin exhibited acceptable biocompatibility on hDPCs. Thus, apigeinin may affect adherence and biofilm formation by altering the surface properties of S. mutans without obvious adverse effect on hDPCs.
柚皮素是一种类黄酮，具有抗龋特性。细菌黏附是形成稳定生物膜导致龋齿的初始步骤。细菌黏附受表面特性的影响，包括疏水性和细菌聚集。然而，柚皮素对致龋菌表面特性的影响尚未报道。本研究旨在探讨柚皮素对变异链球菌 UA159 黏附和生物膜形成的影响。还测定了疏水性、细菌聚集、pac 和 gbpC 基因表达以及对人牙髓细胞的细胞毒性。柚皮素显著抑制了变异链球菌的黏附和生物膜形成。与对照组相比，疏水性降低，而聚集率显著增加。柚皮素显著抑制 pac 和 gbpC 基因表达。柚皮素对 hDPCs 表现出良好的生物相容性。因此，柚皮素可能通过改变 S. mutans 的表面特性来影响其黏附和生物膜形成，而对 hDPCs 没有明显的不良影响。
