Mazurov A V, Prokazova N V, Mikhailenko I A, Mukjin D N, Repin V S, Bergelson L D
Institute of Experimental Cardiology, Academy of Medical Sciences of the U.S.S.R., Moscow.
Biochim Biophys Acta. 1988 Feb 22;968(2):167-71. doi: 10.1016/0167-4889(88)90004-3.
Platelet interaction with gangliosides GD3, GM3, GM1, GD1a and GT1b has been investigated. These gangliosides were previously identified in the vessel wall and ganglioside GD3 was found to accumulate selectively in the intima of atherosclerotic vessels. Gangliosides were adsorbed to plastic and incubated with 51Cr-labeled platelets. The adhesion of gel-filtered platelets to ganglioside GD3 was 3-4 times higher than to other immobilized gangliosides and to albumin-treated plastic. As was shown by scanning electron microscopy, GD3 stimulated intensive spreading of adherent platelets and formation of surface-bound aggregates, while only single unspread platelets were present on the surfaces coated with other gangliosides. GD3 isolated from milk and from human aorta possess the same stimulating activity. Platelet adhesion to GD3 decreased significantly in the presence of the stable prostacyclin analogue, carbacyclin.
已对血小板与神经节苷脂GD3、GM3、GM1、GD1a和GT1b的相互作用进行了研究。这些神经节苷脂先前在血管壁中被鉴定出来，并且发现神经节苷脂GD3选择性地积聚在动脉粥样硬化血管的内膜中。将神经节苷脂吸附到塑料上，并与51Cr标记的血小板一起孵育。经凝胶过滤的血小板与神经节苷脂GD3的黏附力比与其他固定化神经节苷脂以及白蛋白处理过的塑料的黏附力高3至4倍。扫描电子显微镜显示，GD3刺激黏附血小板的强烈铺展并形成表面结合的聚集体，而在涂有其他神经节苷脂的表面上仅存在单个未铺展的血小板。从牛奶和人主动脉中分离出的GD3具有相同的刺激活性。在稳定的前列环素类似物卡巴前列素存在的情况下，血小板对GD3的黏附显著降低。
