Jennemann R, Wiegandt H
Physiologisch-Chemisches Institut, Philipps-Universität, Marburg, Germany.
Lipids. 1994 May;29(5):365-8. doi: 10.1007/BF02537191.
A method is described for the preparation of ganglioside Glac2 [(II3(NeuAc)2-LacCer, GD3] from cream of bovine milk using liquid-phase extraction with methanol or ethanol followed by anion exchange chromatography. The method is rapid and inexpensive; 1 kg cream, centrifuged from 14-15 L of bovine milk, yields approximately 70 mg of pure ganglioside Glac2. The sialic acid constituent of ganglioside Glac2 isolated from bovine milk cream consists solely of the N-acetylneuraminic acid derivative. The major components of its ceramide consist of octadecasphing-4-enine and the 22:0 (behenic acid) and 23:0 fatty acids.
描述了一种从牛乳奶油中制备神经节苷脂Glac2 [（II3（NeuAc）2-LacCer，GD3]的方法，该方法采用甲醇或乙醇进行液相萃取，然后进行阴离子交换色谱法。该方法快速且成本低廉；从14-15升牛乳中离心得到的1千克奶油可产生约70毫克的纯神经节苷脂Glac2。从牛乳奶油中分离出的神经节苷脂Glac2的唾液酸成分仅由N-乙酰神经氨酸衍生物组成。其神经酰胺的主要成分包括十八碳-4-烯鞘氨醇以及22:0（山嵛酸）和23:0脂肪酸。
