Department of Biochemistry, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
Department of Biochemistry, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa; Central Analytical Facility, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
J Pharm Biomed Anal. 2021 Feb 20;195:113846. doi: 10.1016/j.jpba.2020.113846. Epub 2021 Jan 7.
Ion mobility spectrometry (IMS) is a rapid separation technique capable of extracting complementary structural information to chromatography and mass spectrometry (MS). IMS, especially in combination with MS, has experienced inordinate growth in recent years as an analytical technique, and elicited intense interest in many research fields. In natural product analysis, IMS shows promise as an additional tool to enhance the performance of analytical methods used to identify promising drug candidates. Potential benefits of the incorporation of IMS into analytical workflows currently used in natural product analysis include the discrimination of structurally similar secondary metabolites, improving the quality of mass spectral data, and the use of mobility-derived collision cross-section (CCS) values as an additional identification criterion in targeted and untargeted analyses. This review aims to provide an overview of the application of IMS to natural product analysis over the last six years. Instrumental aspects and the fundamental background of IMS will be briefly covered, and recent applications of the technique for natural product analysis will be discussed to demonstrate the utility of the technique in this field.
离子迁移谱（IMS）是一种快速分离技术，能够提取与色谱和质谱（MS）互补的结构信息。IMS 尤其是与 MS 结合使用，近年来作为一种分析技术经历了异常的增长，并在许多研究领域引起了强烈的兴趣。在天然产物分析中，IMS 有望成为一种额外的工具，以提高用于鉴定有前途的药物候选物的分析方法的性能。将 IMS 纳入天然产物分析中当前使用的分析工作流程的潜在好处包括区分结构相似的次生代谢产物，改善质谱数据的质量，以及使用迁移率衍生的碰撞截面（CCS）值作为靶向和非靶向分析中的附加鉴定标准。本综述旨在概述过去六年来 IMS 在天然产物分析中的应用。将简要介绍仪器方面和 IMS 的基本背景，并讨论该技术在天然产物分析中的最新应用，以证明该技术在该领域的实用性。
