Chen Dan, Li Yue, Sun Han, Xiao Meng, Zhang Rui Li, Qiu Ling, Tan Bei, Qian Jia Ming
Department of Gastroenterology, PUMC Hospital, CAMS and PUMC,Beijing 100730,China.
Department of Emergency, PUMC Hospital, CAMS and PUMC,Beijing 100730,China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2020 Dec 30;42(6):740-748. doi: 10.3881/j.issn.1000-503X.12105.
Objective To investigate the correlation between serum total 25-hydroxyvitamin D[T-25(OH)D]level and fecal microbiota in patients with inflammatory bowel disease(IBD). Methods Twenty-three patients with IBD completed the tests for serum T-25(OH)D,and the fecal microbiota was studied using V4 hypervariable region of 16S ribosomal RNA(rRNA)gene sequencing.According to serum T-25(OH)D level,the patients were divided into three groups including vitamin D normal group(=5),vitamin D insufficiency group(=5),and vitamin D deficiency group(=13). Results There was no significant difference between these three groups in Alpha diversity or Beta diversity.Ternary pot at phylum level revealed that the abundance of was the highest in the vitamin D deficiency group and was the highest in the vitamin D sufficiency group.Spearman correlation analysis showed that at the phylum level serum T-25(OH)D level was negatively correlated with the abundance of (=-0.445,=0.033)and positively correlated with the abundance of ( =0.447,=0.033);at family level it was positively correlated with the abundance of ( =0.414,=0.049), ( =0.468,=0.024), ( =0.584,=0.003),and ( =0.507,=0.014)and negatively correlated with the abundance of (=-0.514, =0.012);and at genus level it was positively correlated with the abundance of (=0.459,=0.028), (=0.468,=0.024),unidentified (=0.485,=0.019), (=0.544,=0.007), (=0.475,=0.022), (=0.510,=0.013), (=0.455,=0.029),and (=0.617,=0.002). Conclusions The fecal microbiota composition varies in IBD patients with different serum T-25(OH)D levels.The abundance of increases and the abundance of decreases in IBD patients with vitamin D deficiency compared with IBD patients with normal vitamin D level.Serum T-25(OH)D level is negatively correlated with the abundance of some harmful bacteria(e.g.)but is positively correlated with the abundance of some probiotics such as ,,and .
目的 探讨炎症性肠病（IBD）患者血清总25-羟基维生素D[T-25(OH)D]水平与粪便微生物群之间的相关性。方法 23例IBD患者完成血清T-25(OH)D检测，并采用16S核糖体RNA（rRNA）基因V4高变区测序研究粪便微生物群。根据血清T-25(OH)D水平，将患者分为三组，即维生素D正常组（=5）、维生素D不足组（=5）和维生素D缺乏组（=13）。结果 这三组在α多样性或β多样性方面无显著差异。门水平的三元图显示，维生素D缺乏组中某菌属丰度最高，维生素D充足组中另一菌属丰度最高。Spearman相关性分析表明，在门水平上，血清T-25(OH)D水平与某菌属丰度呈负相关（=-0.445，=0.033），与另一菌属丰度呈正相关（ =0.447，=0.033）；在科水平上，与某菌科丰度呈正相关（ =0.414，=0.049）、另一菌科（ =0.468，=0.024）、又一菌科（ =0.584，=0.003）和再一菌科（ =0.507，=0.014）呈正相关，与某菌科丰度呈负相关（=-0.514， =0.012）；在属水平上，与某菌属丰度呈正相关（=0.459，=0.028）、另一菌属（=0.468，=0.024）、未鉴定菌属（=0.485，=0.019）、又一菌属（=0.544，=0.007）、再一菌属（=0.475，=0.022）、另一菌属（=0.510，=0.013）、又一菌属（=0.455，=0.029）和再一菌属（=0.617，=0.002）呈正相关。结论 不同血清T-25(OH)D水平的IBD患者粪便微生物群组成不同。与维生素D水平正常的IBD患者相比，维生素D缺乏的IBD患者中某菌属丰度增加，另一菌属丰度降低。血清T-25(OH)D水平与一些有害菌（如某菌属）的丰度呈负相关，但与一些益生菌（如某菌属、另一菌属和又一菌属）的丰度呈正相关。
