Xiamen Institute of Union Respiratory Health, Xiamen, China.
Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun, China.
Microb Cell Fact. 2022 Jul 11;21(1):137. doi: 10.1186/s12934-022-01858-6.
Vitamin D insufficiency or deficiency is associated with an altered microbiota in older men. However, the relationship between the gut microbiota and 25-hydroxyvitamin D (25(OH)D) levels remains unknown in postmenopausal women. In this study, fecal microbiota profiles for 88 postmenopausal women in the high 25(OH)D (HVD) group (n = 44) and the low 25(OH)D (LVD) group (n = 44) were determined. An integrated 16S rRNA gene sequencing and liquid chromatography-mass spectrometry (LC-MS)-based metabolomics approach was applied to explore the association of serum 25(OH)D levels with the gut microbiota and fecal metabolic phenotype. Adjustments were made using several statistical models for potential confounding variables identified from the literature.
The results demonstrated that the community diversity estimated by the Observe, Chao1 and ACE indexes was significantly lower in the LVD group than in the HVD group. Additionally, two kinds of characteristic differences in the microflora were analyzed in the HVD group, and ten kinds of characteristic differences in the microflora were analyzed in the LVD group. We observed that some bacteria belonging to the genera Bifidobacterium, Bacillus, F0332 and Gemella, were enriched in the LVD group, as were other genera, including Lachnoclostridium, UC5_1_2E3, Ruminococcus_gnavus_group and un_f_Lachnospiraceae. Christensenellaceae, Eggerthellaceae and Cloacibacillus were enriched in the HVD group. The L-pyroglutamic acid, inosine, and L-homocysteic acid levels were higher in the HVD group and were negatively correlated with the 1,2-benzenedicarboxylic acid and cholic acid metabolic levels.
These observations provide a better understanding of the relationships between serum 25(OH)D levels and the fecal microbiota and metabolites in postmenopausal women.
维生素 D 不足或缺乏与老年男性的肠道菌群改变有关。然而，绝经后妇女的肠道菌群与 25-羟维生素 D（25（OH）D）水平之间的关系尚不清楚。在这项研究中，测定了高 25（OH）D（HVD）组（n=44）和低 25（OH）D（LVD）组（n=44）的 88 名绝经后妇女的粪便微生物群谱。应用整合的 16S rRNA 基因测序和基于液相色谱-质谱（LC-MS）的代谢组学方法，探索血清 25（OH）D 水平与肠道菌群和粪便代谢表型的关联。根据文献中确定的潜在混杂变量，使用几种统计模型进行了调整。
结果表明，LVD 组的观察、Chao1 和 ACE 指数估计的群落多样性明显低于 HVD 组。此外，在 HVD 组中分析了两种微生物群落的特征差异，在 LVD 组中分析了十种微生物群落的特征差异。我们观察到，一些属于双歧杆菌、芽孢杆菌、F0332 和真杆菌属的细菌在 LVD 组中富集，其他属，包括 Lachnoclostridium、UC5_1_2E3、Ruminococcus_gnavus_group 和 un_f_Lachnospiraceae，也在 LVD 组中富集。Christensenellaceae、Eggerthellaceae 和 Cloacibacillus 在 HVD 组中富集。HVD 组的 L-焦谷氨酸、肌苷和 L-同型半胱氨酸水平较高，与 1,2-苯二甲酸和胆酸代谢水平呈负相关。
这些观察结果提供了对绝经后妇女血清 25（OH）D 水平与粪便微生物群和代谢物之间关系的更好理解。
