Mohamed Sara, Elmohamady Mohamed N, Abdelrahman Sohier, Amer Mahmoud M, Abdelhamid Ahmed G
Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.
Ophthalmology Department, Faculty of Medicine, Benha University, Benha 13511, Egypt.
Saudi Pharm J. 2020 Dec;28(12):1558-1565. doi: 10.1016/j.jsps.2020.10.002. Epub 2020 Oct 22.
Conjunctivitis, caused by bacterial infections, represents health concern and diagnosis of the disease is pivotal for the proper selection of the treatment. The main causes of bacterial conjunctivitis vary in different countries. The current study investigated the common bacterial causes of bacterial conjunctivitis from eye clinics' attendants and evaluated the effectiveness of different therapeutic approaches. Eye swabs from patients, diagnosed with conjunctivitis, were assessed microbiologically and the isolated bacteria were identified using the standard biochemical identification and sequencing of the 16S rRNA gene. Antibiotics' susceptibility of the conjunctivitis-associated bacterial pathogens was evaluated against nineteen broad-spectrum antibiotics. In the meanwhile, cell-free preparations from probiotic and strains were used to evaluate their antagonistic activities. Findings from this study showed that out of 52 specimen, 17 eye swabs from patients with conjunctivitis were bacterial culture-positive. The identity of the bacterial species, using the biochemical identification system, was (4 isolates) and (13 isolates). spp. showed susceptibility to linezolid, vancomycin, novobiocin, and fluoroquinolones (norfloxacin, ofloxacin, ciprofloxacin and levofloxacin). However, isolates from the two spp. expressed resistance to penicillin G, oxacillin, and cephalexin. As alternatives to antibiotics, the growth of spp., including isolates with antibiotic resistance, was inhibited by cell-free preparations of the 4 probiotic and the 2 strains. These findings provide evidence that topical antibiotics such as fluoroquinolones are still effective antimicrobial agents against staphylococci associated with conjunctivitis whereas probiotic preparations could be promising for further research to pave the way for their therapeutic applications against ophthalmic diseases.
由细菌感染引起的结膜炎是一个健康问题，对该疾病的诊断对于正确选择治疗方法至关重要。细菌性结膜炎的主要病因在不同国家有所不同。本研究调查了眼科门诊患者细菌性结膜炎的常见细菌病因，并评估了不同治疗方法的有效性。对诊断为结膜炎的患者的眼拭子进行微生物学评估，并使用标准生化鉴定和16S rRNA基因测序来鉴定分离出的细菌。评估了与结膜炎相关的细菌病原体对19种广谱抗生素的药敏性。同时，使用益生菌和菌株的无细胞制剂来评估它们的拮抗活性。本研究结果表明，在52份标本中，17份来自结膜炎患者的眼拭子细菌培养呈阳性。使用生化鉴定系统鉴定的细菌种类为（4株分离菌）和（13株分离菌）。葡萄球菌属对利奈唑胺、万古霉素、新生霉素和氟喹诺酮类（诺氟沙星、氧氟沙星、环丙沙星和左氧氟沙星）敏感。然而，来自两种葡萄球菌属的分离菌对青霉素G、苯唑西林和头孢氨苄耐药。作为抗生素的替代品，4种益生菌和2种菌株的无细胞制剂抑制了包括耐药分离菌在内的葡萄球菌属的生长。这些发现提供了证据，表明氟喹诺酮类等局部抗生素仍然是对抗与结膜炎相关的葡萄球菌的有效抗菌剂，而益生菌制剂有望进一步研究，为其在眼科疾病中的治疗应用铺平道路。
