六种唇形科植物不同提取物对在无细胞、原核和真核模型中评估的氧化应激有害影响的作用

The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models .

作者信息

Oalđe Mariana M, Kolarević Stoimir M, Živković Jelena C, Vuković-Gačić Branka S, Jovanović Marić Jovana M, Kračun Kolarević Margareta J, Đorđević Jelena Z, Alimpić Aradski Ana Z, Marin Petar D, Šavikin Katarina P, Duletić-Laušević Sonja N

机构信息

University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac", Studentski trg, 16, Belgrade, Serbia.

University of Belgrade, Faculty of Biology, Chair of Microbiology, Centre for Genotoxicology and Ecogenotoxicology, Studentski trg, 16, Belgrade, Serbia.

出版信息

Saudi Pharm J. 2020 Dec;28(12):1592-1604. doi: 10.1016/j.jsps.2020.10.006. Epub 2020 Oct 24.

DOI:10.1016/j.jsps.2020.10.006

PMID:33424252

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7783233/

Abstract

The main objective of this research was to evaluate the impact of methanolic, ethanolic and aqueous extracts of L., L., L., L., L. and L. (Lamiaceae) on the effects of free radicals using different model systems. The extracts were characterized on the basis of the contents of total phenolics, phenolic acids, flavonoids and flavonols, and also using high-performance liquid chromatography with diode-array detection. Antioxidant activity was assessed using DPPH assay. The genoprotective properties were tested using plasmid relaxation assay on pUC19 XL1-Blue, while SOS/ assay on TA1535/pSK1002 and Comet assay on human lung fibroblasts were used to assess the antigenotoxicity of the extracts. Ethanolic extracts had the most phenolics (up to 236.20 mg GAE/g at 0.5 mg/mL), flavonoids (up to 42.47 mg QE/g at 0.5 mg/mL) and flavonols (up to 16.56 mg QE/g at 0.5 mg/mL), and they exhibited the highest DPPH activity (up to 92.16% at 0.25 mg/mL). Interestingly enough, aqueous extracts provided the best protection of plasmid DNA (the lowest IC value was 0.17 mg/mL). Methanolic extracts, on the other hand, most efficiently protected the prokaryotic DNA, while all the extracts had a significant impact against genomic damages inflicted on human fibroblasts. extracts are considered to be the most promising in preserving the overall DNA integrity against oxidative genomic damages. Moreover, HPLC-DAD analysis highlighted rosmarinic acid as the most abundant in the investigated samples (551.45 mg/mL in total in all the extracts), followed by luteolin-7--glucoside (150.19 mg/mL in total), while their presence correlates with most of the displayed activities. The novelty of this study is reflected in the application of a prokaryotic model for testing the antigenotoxic effects of Lamiaceae species, as no previous reports have yet been published on the genoprotective potential of these species.

摘要

本研究的主要目的是利用不同的模型系统，评估唇形科植物罗勒属、迷迭香属、薄荷属、留兰香属、薰衣草属和百里香属植物的甲醇提取物、乙醇提取物和水提取物对自由基的影响。基于总酚、酚酸、黄酮类化合物和黄酮醇的含量，以及使用配备二极管阵列检测的高效液相色谱法对提取物进行了表征。使用DPPH法评估抗氧化活性。使用pUC19 XL1-Blue的质粒松弛试验测试基因保护特性，而使用TA1535/pSK1002的SOS/试验和人肺成纤维细胞的彗星试验评估提取物的抗遗传毒性。乙醇提取物的酚类物质含量最高（在0.5 mg/mL时高达236.20 mg GAE/g）、黄酮类化合物含量最高（在0.5 mg/mL时高达42.47 mg QE/g）和黄酮醇含量最高（在0.5 mg/mL时高达16.56 mg QE/g），并且它们表现出最高的DPPH活性（在0.25 mg/mL时高达92.16%）。有趣的是，水提取物对质粒DNA的保护效果最佳（最低IC值为0.17 mg/mL）。另一方面，甲醇提取物最有效地保护了原核DNA，而所有提取物对人成纤维细胞遭受的基因组损伤都有显著影响。提取物被认为在保护整体DNA完整性免受氧化基因组损伤方面最具前景。此外，HPLC-DAD分析突出显示迷迭香酸是所研究样品中含量最丰富的（所有提取物中总量为551.45 mg/mL），其次是木犀草素-7-O-葡萄糖苷（总量为150.19 mg/mL），而它们的存在与大多数所显示的活性相关。本研究的新颖之处在于应用原核模型来测试唇形科植物的抗遗传毒性作用，因为此前尚未有关于这些植物基因保护潜力的报道发表。