Oalđe Pavlović Mariana, Kolarević Stoimir, Đorđević Jelena, Jovanović Marić Jovana, Lunić Tanja, Mandić Marija, Kračun Kolarević Margareta, Živković Jelena, Alimpić Aradski Ana, Marin Petar D, Šavikin Katarina, Vuković-Gačić Branka, Božić Nedeljković Biljana, Duletić-Laušević Sonja
Department of Plant Morphology and Systematics, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia.
Centre for Genotoxicology and Ecogenotoxicology, Department of Microbiology, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade, Studentski trg 16, 11070 Belgrade, Serbia.
Plants (Basel). 2021 Oct 27;10(11):2306. doi: 10.3390/plants10112306.
This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of , × , , , , and (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 XL1-blue, and the antigenotoxicity on TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7--glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
本研究旨在评估唇形科植物迷迭香（Rosmarinus officinalis L.）、薄荷（Mentha piperita L.）、留兰香（Mentha spicata L.）、牛至（Origanum vulgare L.）、百里香（Thymus vulgaris L.）和罗勒（Ocimum basilicum L.）的甲醇提取物、乙醇提取物和水提取物在不同模型系统中的基因保护、抗诱变以及抗肿瘤潜力。采用分光光度法和高效液相色谱 - 二极管阵列检测（HPLC - DAD）技术对这些提取物中的多酚进行定量，同时使用DPPH法评估抗氧化活性。在pUC19 / XL1 - blue上测试基因保护潜力，在TA1535 / pSK1002和人肺成纤维细胞上测试抗诱变活性，而在结肠癌细胞上评估抗肿瘤活性。迷迭香酸、槲皮素、芦丁和木犀草素 - 7 - O - 葡萄糖苷是鉴定出的化合物。甲醇提取物具有最佳的DPPH清除和SOS诱导活性，而乙醇提取物表现出最高的抗诱变活性。此外，所有提取物对质粒DNA均表现出基因保护潜力。抗肿瘤作用是通过调节活性氧（ROS）、一氧化氮（NO）的产生以及对肿瘤细胞表现出遗传毒性作用来介导的，尤其是乙醇提取物。总体而言，所研究的提取物能够为无细胞、原核和正常人类DNA提供抗氧化保护，同时还能调节肿瘤细胞中ROS和NO的产生，导致对这些细胞的遗传毒性并使其增殖减少。
