El-Borm Hend T, Gobara Marwa S, Badawy Gamal M
Vertebrates, Comparative Anatomy and Embryology-Zoology Department-Faculty of Science-Menoufia University, Egypt.
Zoology Department-Faculty of Science-Menoufia University, Egypt.
Saudi J Biol Sci. 2021 Jan;28(1):440-447. doi: 10.1016/j.sjbs.2020.10.027. Epub 2020 Oct 27.
Labetalol is a medication used to treat maternal hypertension during pregnancy. However, it is often associated with many side effects. Recently, several studies have been focused on the protective effect of medicinal plant extracts, such as ginger, against drugs inducing toxicity. Therefore, it has been hypothesized that ginger aqueous extraction can ameliorate labetalol-induced histological, ultrastructural changes, DNA damage, and apoptosis in fetal heart tissue. To achieve the aim of this study, sixty pregnant female albino rats were divided into 4 groups (15 each). Group I (Control). Group II received ginger (200 mg/kg). Group III received labetalol (300 mg/kg). Group IV received labetalol first followed by ginger. All groups were orally injected daily during the organogenesis phase of gestation i.e., from the 6th to the 15th day, and sacrificed at the 20th day of gestation. Results showed that labetalol-induced marked histological and ultrastructural alterations. Also, there was severe DNA damage and an increase in the apoptotic rates determined by Annexin-V/PI dual staining assay. Injection of the ginger aqueous extract caused evident improvement in cardiac tissue, DNA damage, and apoptotic rates. In conclusion, the results suggest that ginger extract could be a potential candidate agent for reducing labetalol-induced cardiotoxicity in the fetal heart of albino rats.
拉贝洛尔是一种用于治疗孕期母体高血压的药物。然而，它常常伴有许多副作用。最近，几项研究聚焦于药用植物提取物（如生姜）对药物诱导毒性的保护作用。因此，有人提出假设，生姜水提取物可以改善拉贝洛尔诱导的胎儿心脏组织的组织学、超微结构变化、DNA损伤和细胞凋亡。为实现本研究目的，将60只怀孕的雌性白化大鼠分为4组（每组15只）。第一组（对照组）。第二组给予生姜（200毫克/千克）。第三组给予拉贝洛尔（300毫克/千克）。第四组先给予拉贝洛尔，随后给予生姜。所有组在妊娠器官形成期（即从第6天至第15天）每天经口注射给药，并在妊娠第20天处死。结果显示，拉贝洛尔引起明显的组织学和超微结构改变。此外，存在严重的DNA损伤，并且通过膜联蛋白V/碘化丙啶双重染色法测定的凋亡率增加。注射生姜水提取物使心脏组织、DNA损伤和凋亡率有明显改善。总之，结果表明生姜提取物可能是一种潜在的药物，可减轻拉贝洛尔对白化大鼠胎儿心脏的心脏毒性。
