Onbasli Dilsad, Yuvali Gokcen
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey.
Saudi J Biol Sci. 2021 Jan;28(1):478-483. doi: 10.1016/j.sjbs.2020.10.031. Epub 2020 Oct 28.
In this study was conducted the in vitro antimicrobial, antibiofilm, antioxidant, antigenotoxic and anticancer activities investigations on the moss Hedw (). Antimicrobial and antibiofilm activity were tested by MIC and microplate biofilm methods on antibiotic resistant bacteria. While the antioxidant activity of the extract was evaluated by DPPH, metal chelating, plasma lipid peroxidation and total phenolic content, the antigenotoxicity and cytotoxicity were established by Comet test and the WST-1 Cell proliferation assay kit respectively. The MIC values were found to be ≥ 125 µg.mL and a biofilm inhibition of 3-5% against only was observed. Total phenolic compounds were determined as 23.26 mg/g. The results of DPPH assay, chelating and plasma lipid peroxidation activity were found to be 15%, 3% and 4% respectively. The extract was observed to decrease the affect of HO that cause DNA damage. The was also determined 60 ± 5% anticancer activity against SKBR 3 and 76 ± 5% anticancer activity against HeLa cells, where this concentration had only 18 ± 5% cytotoxicity against MCF-12A cells. Also, these results have indicated the potential of for the first time in novel natural compounds search.
本研究对苔藓Hedw（）进行了体外抗菌、抗生物膜、抗氧化、抗基因毒性和抗癌活性研究。采用MIC和微孔板生物膜法对抗生素耐药菌进行抗菌和抗生物膜活性测试。提取物的抗氧化活性通过DPPH、金属螯合、血浆脂质过氧化和总酚含量进行评估，抗基因毒性和细胞毒性分别通过彗星试验和WST - 1细胞增殖检测试剂盒确定。发现MIC值≥125µg/mL，仅对观察到3 - 5%的生物膜抑制率。总酚化合物含量测定为23.26mg/g。DPPH测定、螯合和血浆脂质过氧化活性结果分别为15%、3%和4%。观察到提取物可降低导致DNA损伤的HO的影响。还测定了对SKBR 3有60±5%的抗癌活性，对HeLa细胞有76±5%的抗癌活性，而该浓度对MCF - 12A细胞仅有18±5%的细胞毒性。此外，这些结果首次表明了在新型天然化合物搜索中的潜力。
