Alhimaidi Ahmad R, Ammari Aiman A, Alghadi Muath Q, Al Saiady Mohammed Y, Amran Ramzi A, Swelum Ayman A
King Saud University, College of Science, Zoology Dept, Riyadh, Saudi Arabia.
Thamar University, College of Veterinary Medicine, Department of Histology and Embryology, Dhamar, Yemen.
Saudi J Biol Sci. 2021 Jan;28(1):680-684. doi: 10.1016/j.sjbs.2020.10.059. Epub 2020 Nov 4.
Several methods have been conducted for embryo sex preselection, which includes X- and Y- sperm separation, changing the pH of the female reproductive tract, time of mating before or after ovulation, and feeding formula, such as altering the presence of minerals in diet content before breeding may affect the embryo sex preselection ratio. In this study, three food formulas to feed female sheep were created with the cooperation of the Arabian Agricultural Services Company (Arasco). Ewes were fed with modified food formulas for one month before mating with males. The first group (A) (30 ewes), modified for male embryo gender preselection, were fed a diet with an increased percentage of the minerals Na, K and P. The second group (B) (30 ewes), modified for female sex preselection, were fed a diet with an increased percentage of the minerals Ca and Mg. The third (control) group (C) (30 ewes) were fed the regular (Wafi) food formula. Our results showed no significant differences were in mean body weights between the three groups at the end of the feeding period. The results of different feeding formulas on mineral serum blood samples of ewes showed an increase in Na, K and Cl ions in the serum of group (A) compared to the other groups (B and C). The concentration of Na in the serum of group (A) was significantly (P < 0.05) higher than group (C). The concentration of Cl ions in serum samples of ewes in group (A) was significantly higher than group (C) and group (B) (P < 0.05). The role of maternal feeding on embryo sex preselection shows that the pregnancy rate of animals in group (A) was 73.33%. Group (A) birthed 17 males and 5 females (77.27% and 22.72%, respectively). The pregnancy rate in group (B) was 70%. Group (B) birthed 6 males (27.27%) and 16 females (72.72%). Finally, the control group (C) had a pregnancy rate of 76.66%. They birthed 13 males (54.41%) and 11 females (44.83%). The results of our study confirm that altering the percentage of minerals in the maternal diet plays a role in sex preselection in sheep, which agree with other mammalian studies in rats and mice. Thus, the result of this study can help farmers to manage their breeding. We recommend that more studies on the relationship between minerals in the diet should be conducted for other spices and human sex preselection.
已经开展了几种胚胎性别预选方法，包括X和Y精子分离、改变雌性生殖道的pH值、排卵前后的交配时间以及喂养配方，例如在繁殖前改变饮食中矿物质的含量可能会影响胚胎性别预选比例。在本研究中，与阿拉伯农业服务公司（Arasco）合作创建了三种喂养母羊的食物配方。母羊在与公羊交配前一个月喂食改良后的食物配方。第一组（A组）（30只母羊），为预选雄性胚胎性别而改良，喂食的饮食中钠、钾和磷的矿物质百分比增加。第二组（B组）（30只母羊），为预选雌性胚胎性别而改良，喂食的饮食中钙和镁的矿物质百分比增加。第三组（对照组）（C组）（30只母羊）喂食常规（瓦菲）食物配方。我们的结果表明，在喂养期结束时，三组母羊的平均体重没有显著差异。不同喂养配方对母羊血清矿物质血样的结果显示，与其他组（B组和C组）相比，A组血清中的钠、钾和氯离子增加。A组血清中的钠浓度显著高于C组（P<0.05）。A组母羊血清样本中的氯离子浓度显著高于C组和B组（P<0.05）。母体喂养对胚胎性别预选的作用表明，A组动物的妊娠率为73.33%。A组产下17只雄性和5只雌性（分别为77.27%和22.72%）。B组的妊娠率为70%。B组产下6只雄性（27.27%）和16只雌性（72.72%）。最后，对照组（C组）的妊娠率为76.66%。它们产下13只雄性（54.41%）和11只雌性（44.83%）。我们的研究结果证实，改变母体饮食中矿物质的百分比在绵羊性别预选中起作用，这与对大鼠和小鼠的其他哺乳动物研究结果一致。因此，本研究结果可以帮助农民进行繁殖管理。我们建议针对其他物种和人类性别预选，对饮食中矿物质之间的关系开展更多研究。
