Fu'adil Amin Muhammad Hilman, Lee Soo Rin, Irawan Bambang, Andriyono Sapto, Kim Hyun-Woo
Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan, Republic of Korea.
Department of Biology, Faculty of Science and Technology, Advance Tropical Biodiversity, Genomics, and Conservation Research Group, Universitas Airlangga, Surabaya, Indonesia.
Mitochondrial DNA B Resour. 2021 Mar 16;6(3):953-955. doi: 10.1080/23802359.2021.1889415.
The first mitochondrial genome of was determined by the combination of next-generation sequencing (NGS) and Sanger sequencing methods. A complete circular mitogenome of (16,529 bp) consisted of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two non-coding regions, including a control region (D-loop) and a light strand origin of replication (O). Two start codons (ATG and GTG) and four stop codons (TAG, TAA, TA-, and T-) were used in all the PCGs. Except for ND6 and eight transfer RNAs (tRNAs), all the other genes were encoded in the heavy strand. Based on phylogenetic analysis, formed a clade with three other species in the subfamily Butinae, while the other 10 made a subfamily Eleotrinae clade.
通过下一代测序（NGS）和桑格测序方法相结合，测定了首个[物种名称]的线粒体基因组。一个完整的[物种名称]环状线粒体基因组（16,529 bp）由13个蛋白质编码基因、22个转运RNA、两个核糖体RNA和两个非编码区组成，包括一个控制区（D环）和一个轻链复制起点（O）。所有蛋白质编码基因使用了两个起始密码子（ATG和GTG）和四个终止密码子（TAG、TAA、TA-和T-）。除了ND6和八个转运RNA（tRNA）外，所有其他基因都在重链上编码。基于系统发育分析，[物种名称]与Butinae亚科中的其他三个物种形成一个分支，而其他10个物种形成Eleotrinae亚科分支。
