Tajabadi Elham, Javadi Abdolreza, Azar Nasim Ahmadi, Najafi Masoud, Shirazi Alireza, Shabeeb Dheyauldeen, Musa Ahmed Eleojo
Department of Medical Radiation Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Pathology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Int J Reprod Biomed. 2020 Dec 21;18(12):1073-1080. doi: 10.18502/ijrm.v18i12.8029. eCollection 2020 Dec.
The spermatogenesis system includes highly radiosensitive cells. Hence, this system is a potential target for toxic effects of ionizing radiation during radiotherapy of abdomen and pelvis cancers, as well as after accidental radiation events. Accordingly, metformin and melatonin are two important radioprotectors that have shown an ability to prevent cell death through neutralization of free radicals and stimulating DNA damage responses.
To evaluate the radioprotective effects of melatonin and metformin on mice spermatogenesis when administered alone or as a combination.
In this histological Study, 40 (6-8 wk, 30 gr) NMRI mice were divided into 8 groups (n = 5/each) as control, metformin, melatonin, melatonin + metformin, radiation, radiation + melatonin, radiation + metformin, and radiation + melatonin + metformin. 37 days after the irradiation, the testicular tissues were collected for histological evaluation.
Single administration of melatonin could ameliorate effectively radiation toxicity in mice testis. Metformin showed radioprotective effects on some parameters such as the numbers of spermatogonia and mature sperms. Interestingly, the melatonin and metformin combination reversed the reduced number of sperms rather than single drug administration.
The combination of melatonin with metformin can protect mice spermatogenesis against ionizing radiation more effectively compared to the single forms of these drugs.
精子发生系统包含高度放射敏感细胞。因此，该系统是腹部和盆腔癌症放疗期间以及意外辐射事件后电离辐射毒性作用的潜在靶点。相应地，二甲双胍和褪黑素是两种重要的辐射防护剂，已显示出能够通过中和自由基和刺激DNA损伤反应来预防细胞死亡的能力。
评估单独使用或联合使用褪黑素和二甲双胍对小鼠精子发生的辐射防护作用。
在这项组织学研究中，将40只（6 - 8周龄，30克）NMRI小鼠分为8组（每组n = 5），分别为对照组、二甲双胍组、褪黑素组、褪黑素 + 二甲双胍组、辐射组、辐射 + 褪黑素组、辐射 + 二甲双胍组和辐射 + 褪黑素 + 二甲双胍组。照射37天后，收集睾丸组织进行组织学评估。
单独给予褪黑素可有效减轻小鼠睾丸的辐射毒性。二甲双胍对某些参数如精原细胞数量和成熟精子数量显示出辐射防护作用。有趣的是，与单独给药相比，褪黑素和二甲双胍联合使用可逆转精子数量的减少。
与这些药物的单一形式相比，褪黑素与二甲双胍联合使用能更有效地保护小鼠精子发生免受电离辐射的影响。
