Arıcıgil Mitat, Dündar Mehmet Akif, Yücel Abitter, Eryılmaz Mehmet Akif, Aktan Meryem, Alan Mehmet Akif, Fındık Sıdıka, Kılınç İbrahim
a Department of Otorhinolaryngology , Meram Faculty of Medicine, Necmettin Erbakan University , Konya , Turkey.
b Department of Otorhinolaryngology , Horasan State Hospital , Erzurum , Turkey.
Int J Radiat Biol. 2017 Dec;93(12):1350-1356. doi: 10.1080/09553002.2017.1397296. Epub 2017 Nov 20.
We aimed to investigate the protective effect of melatonin in radiotherapy-induced thyroid gland injury in an experimental rat model.
Thirty-two rats were divided into four groups: the control group, melatonin treatment group, radiotherapy group and melatonin plus radiotherapy group. The neck region of each rat was defined by simulation and radiated with 2 Gray (Gy) per min with 6-MV photon beams, for a total dose of 18 Gy. Melatonin was administered at a dose of 50 mg/kg through intraperitoneal injection, 15 min prior to radiation exposure. Thirty days after the beginning of the study, rats were decapitated and analyses of blood and thyroid tissue were performed.
Tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) levels in the radiotherapy group were significantly higher than those in the melatonin plus radiotherapy group (p < .05), whereas interleukin-10 (IL-10) and glutathione (GSH) values were higher in the melatonin plus radiotherapy group (p < .05). The infiltration of inflammatory cells and percentage of apoptosis in the radiotherapy group were significantly higher than those in the melatonin plus radiotherapy group (p < .05).
Melatonin helped protect thyroid gland structure against the undesired cytotoxic effects of radiotherapy in rats.
我们旨在研究褪黑素在实验性大鼠模型中对放疗所致甲状腺损伤的保护作用。
32只大鼠分为四组：对照组、褪黑素治疗组、放疗组和褪黑素加放疗组。通过模拟确定每只大鼠的颈部区域，并用6兆伏光子束以每分钟2戈瑞（Gy）的剂量进行照射，总剂量为18 Gy。在辐射暴露前15分钟，通过腹腔注射以50毫克/千克的剂量给予褪黑素。研究开始30天后，将大鼠断头并进行血液和甲状腺组织分析。
放疗组中的肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、硫代巴比妥酸反应物质（TBARS）和一氧化氮（NO）水平显著高于褪黑素加放疗组（p < 0.05），而褪黑素加放疗组中的白细胞介素-10（IL-10）和谷胱甘肽（GSH）值更高（p < 0.05）。放疗组中炎症细胞的浸润和凋亡百分比显著高于褪黑素加放疗组（p < 0.05）。
褪黑素有助于保护大鼠甲状腺结构免受放疗产生的不良细胞毒性作用。
