Institute of Microbial Biotechnology, Agricultural University of Georgia, 240 David Aghmashenebeli alley, 0131, Tbilisi, Georgia.
Arch Microbiol. 2021 Sep;203(7):3905-3917. doi: 10.1007/s00203-021-02374-8. Epub 2021 May 20.
The present study aimed to identify a pair of fungal strains that promote laccase production in the co-cultivation of white-rot basidiomycetes and to determine the optimum conditions to enhance enzyme synthesis under co-fermentation of mandarin peels. Co-cultivation of Cerrena unicolor with Trametes versicolor, Lenzites betulina, and Panus lecomtei led to up-regulation of laccase activity. Moreover, interspecific interaction of Cerrena unicolor and Trametes versicolor induced the production of two new laccase isoenzymes. By contrast, interactions of Cerrena unicolor with Trametes coccineus and Trametes hirsuta resulted in a multiple decreased ability of Cerrena unicolor to produce laccase. Co-cultivation of Cerrena unicolor with other fungi 3- to 12-fold down-regulated manganese peroxidase (MnP) activity. The outcomes of these fungal interactions are closely related to the initial concentration and availability of the nutrients, the partners' inoculum ratio, time, and sequence of their inoculation. Co-cultivation of Cerrena unicolor and Trametes versicolor in fermenter resulted in the accumulation of 476 U/mL laccase and 1.12 U/mL MnP.
本研究旨在鉴定一对真菌菌株，以促进白腐菌共生培养中漆酶的产生，并确定在共发酵处理柑橘皮时，提高酶合成的最佳条件。在彩绒革盖菌与变色栓菌、木蹄层孔菌和粗毛栓菌的共培养中，漆酶活性得到上调。此外，彩绒革盖菌与变色栓菌的种间相互作用诱导了两种新的漆酶同工酶的产生。相比之下，彩绒革盖菌与密粘褶菌和粗毛栓菌的相互作用导致彩绒革盖菌产生漆酶的能力多次下降。彩绒革盖菌与其他真菌的共培养将锰过氧化物酶（MnP）活性降低了 3 至 12 倍。这些真菌相互作用的结果与初始浓度和养分的可用性、接种物的接种比例、时间和接种顺序密切相关。彩绒革盖菌和变色栓菌在发酵罐中的共培养导致漆酶积累 476 U/mL，MnP 积累 1.12 U/mL。
