George F W, Peterson K G
Department of Cell Biology and Anatomy, University of Texas Health Science Center, Dallas 75235.
Endocrinology. 1988 Mar;122(3):1159-64. doi: 10.1210/endo-122-3-1159.
To determine the role of 5 alpha-dihydrotestosterone (DHT; 17 beta-hydroxy-5 alpha-androstan-3-one) in formation of the embryonic prostate, we quantitated prostatic development in urogenital tracts of control male newborn and offspring of rats treated with a specific 5 alpha-reductase inhibitor (L652,931; Merck, Sharp, and Dohme) during prostate morphogenesis. Treatment with the 5 alpha-reductase inhibitor (50 mg/kg.day) from days 14-22 of gestation impaired development of the prostate and virilization of the external genitalia in male offspring compared to those in control animals. However, virilization of the internal genitalia (seminal vesicles, epididymis) was unaffected. Simultaneous administration of DHT (50 mg/kg.day) with the 5 alpha-reductase inhibitor restored prostate development and anogenital distances of males to normal and virilized the external genitalia of females. We conclude that DHT is the active androgen responsible for prostatic development in the rat.
为了确定5α-二氢睾酮（DHT；17β-羟基-5α-雄甾烷-3-酮）在胚胎前列腺形成中的作用，我们对对照雄性新生大鼠以及在前列腺形态发生期间用特定的5α-还原酶抑制剂（L652,931；默克公司）处理的大鼠后代的泌尿生殖道中的前列腺发育进行了定量分析。与对照动物相比，在妊娠第14至22天用5α-还原酶抑制剂（50毫克/千克·天）处理会损害雄性后代前列腺的发育以及外生殖器的男性化。然而，内生殖器（精囊、附睾）的男性化未受影响。将DHT（50毫克/千克·天）与5α-还原酶抑制剂同时给药可使雄性的前列腺发育和肛门生殖器距离恢复正常，并使雌性的外生殖器男性化。我们得出结论，DHT是负责大鼠前列腺发育的活性雄激素。
