College of Plant Protection, China Agricultural University, Haidian District, Beijing, 100193, People's Republic of China.
College of Horticulture, China Agricultural University, Haidian District, Beijing, 100193, People's Republic of China.
Arch Virol. 2021 Mar;166(3):973-976. doi: 10.1007/s00705-020-04937-4. Epub 2021 Jan 11.
In this study, a new double-stranded RNA (dsRNA) virus, Alternaria tenuissima partitivirus 1 (AttPV1), was isolated from Alternaria tenuissima strain XJ-BZ-2-6, a phytopathogenic fungus infecting cotton in China. The genome of AttPV1 comprised three dsRNAs of 1,785 nt (dsRNA1), 1,545 nt (dsRNA2), and 1,537 nt (dsRNA3) in length, the nucleotide sequence of which was determined using reverse transcription polymerase chain reaction, random-primed clones, and RNA-ligase-mediated rapid amplification of cDNA ends. dsRNA1 had a single open reading frame encoding a putative 61.54-kDa RNA-dependent RNA polymerase (RdRp). dsRNA2 and dsRNA3 were predicted to encode putative coat proteins (CPs) of 47.90 kDa and 46.25 kDa, respectively. The RdRp domain shared 63.54-73.17% amino acid sequence identity with members of the genus Gammapartitivirus. Phylogenetic trees based on RdRp or CP sequences showed that AttPV1 clustered with members of the genus Gammapartitivirus. Hence, these results indicate that AttPV1 is a new gammapartitivirus from A. tenuissima.
在本研究中，从一种感染中国棉花的植物病原菌链格孢菌菌株 XJ-BZ-2-6 中分离到一种新的双链 RNA(dsRNA)病毒，即细极链格孢短小病毒 1(AttPV1)。AttPV1 的基因组由 3 条 dsRNA 组成，分别长 1785 nt(dsRNA1)、1545 nt(dsRNA2)和 1537 nt(dsRNA3)，其核苷酸序列通过反转录聚合酶链反应、随机引物克隆和 RNA 连接酶介导的 cDNA 末端快速扩增确定。dsRNA1 具有一个单一的开放阅读框，编码一个假定的 61.54kDa RNA 依赖性 RNA 聚合酶(RdRp)。dsRNA2 和 dsRNA3 预测分别编码假定的 47.90kDa 和 46.25kDa 的外壳蛋白(CPs)。RdRp 结构域与 Gammapartitivirus 属的成员具有 63.54-73.17%的氨基酸序列同一性。基于 RdRp 或 CP 序列构建的系统进化树表明，AttPV1 与 Gammapartitivirus 属的成员聚类在一起。因此，这些结果表明 AttPV1 是一种来自细极链格孢的新的 gammapartitivirus。
