Biochemistry. 2021 Apr 6;60(13):966-990. doi: 10.1021/acs.biochem.0c00860. Epub 2021 Jan 11.
Self-assembled nanostructures that are sensitive to environmental stimuli are promising nanomaterials for drug delivery. In this class, disulfide-containing redox-sensitive strategies have gained enormous attention because of their wide applicability and simplicity of nanoparticle design. In the context of nucleic acid delivery, numerous disulfide-based materials have been designed by relying on covalent or noncovalent interactions. In this review, we highlight major advances in the design of disulfide-containing materials for nucleic acid encapsulation, including covalent nucleic acid conjugates, viral vectors or virus-like particles, dendrimers, peptides, polymers, lipids, hydrogels, inorganic nanoparticles, and nucleic acid nanostructures. Our discussion will focus on the context of the design of materials and their impact on addressing the current shortcomings in the intracellular delivery of nucleic acids.
自组装的纳米结构对环境刺激敏感，是有前途的药物输送纳米材料。在这一类中，由于其广泛的适用性和纳米粒子设计的简单性，含二硫键的氧化还原敏感策略引起了极大的关注。在核酸递送上，许多基于二硫键的材料都是通过依赖于共价或非共价相互作用来设计的。在这篇综述中，我们重点介绍了用于核酸封装的含二硫键材料的设计方面的主要进展，包括共价核酸缀合物、病毒载体或类病毒颗粒、树枝状大分子、肽、聚合物、脂质、水凝胶、无机纳米粒子和核酸纳米结构。我们的讨论将集中在材料设计的背景及其对解决核酸细胞内递送上当前缺点的影响。
