Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, International Centre for Life, Central Parkway, Newcastle Upon Tyne, NE1 3BZ, UK.
Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, International Centre for Life, Central Parkway, Newcastle Upon Tyne, NE1 3BZ, UK; Renal Services, The Newcastle Upon Tyne Hospitals NHS Foundations Trust, Newcastle Upon Tyne, NE7 7DN, UK; NIHR Newcastle Biomedical Research Centre, Newcastle University, Newcastle Upon Tyne, Tyne and Wear, NE45PL, UK.
Mol Cell Probes. 2021 Apr;56:101694. doi: 10.1016/j.mcp.2021.101694. Epub 2021 Jan 9.
The ability to preserve and transport human cells in a stable medium over long distances is critical to collaborative efforts and the advancement of knowledge in the study of human disease. This is particularly important in the study of rare diseases. Recently, advancements in the understanding of renal ciliopathies has been achieved via the use of patient urine-derived cells (UDCs). However, the traditional method of cryopreservation, although considered as the gold standard, can result in decreased sample viability of many cell types, including UDCs. Delays in transportation can have devastating effects upon the viability of samples, and may even result in complete destruction of cells following evaporation of dry ice or liquid nitrogen, leaving samples in cryoprotective agents, which are cytotoxic at room temperature. The loss of any patient sample in this manner is detrimental to research, however it is even more so when samples are from patients with a rare disease. In order to overcome the associated limitations of traditional practices, new methods of preservation and shipment, including cell encapsulation within hydrogels, and transport in specialised devices are continually being investigated. Here we summarise and compare traditional methods with emerging novel alternatives for the preservation and shipment of cells, and consider the effectiveness of such methods for use with UDCs to further enable the study and understanding of kidney diseases.
在稳定的介质中远距离保存和运输人类细胞的能力对于合作努力和人类疾病研究知识的进步至关重要。这在研究罕见病时尤为重要。最近，通过使用患者尿液来源的细胞（UDC），对肾脏纤毛病的理解取得了进展。然而，传统的冷冻保存方法虽然被认为是金标准，但会导致包括 UDC 在内的许多细胞类型的样本活力下降。运输过程中的延迟会对样本的活力产生毁灭性的影响，甚至可能导致干冰或液氮蒸发后细胞完全破坏，使样本留在细胞保护剂中，而室温下细胞保护剂具有细胞毒性。以这种方式损失任何患者样本都对研究不利，但对于来自罕见病患者的样本更是如此。为了克服传统实践的相关限制，包括在水凝胶内封装细胞和在专用设备中运输在内的新的保存和运输方法正在不断被研究。在这里，我们总结和比较了传统方法与新兴的替代方法，用于保存和运输细胞，并考虑了这些方法在 UDC 中的有效性，以进一步促进对肾脏疾病的研究和理解。
