五味子乙素增强 Celsior 溶液在人脐带间充质干细胞低温保存中的作用。
Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells.
机构信息
General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China.
Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China.
出版信息
Tissue Eng Regen Med. 2023 Jun;20(3):447-459. doi: 10.1007/s13770-023-00531-2. Epub 2023 Mar 22.
BACKGROUND
Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs.
METHODS
hUCMSCs were isolated from Wharton's jelly. Subsequently, hUCMSCs were exposed to cold storage (4 °C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated.
RESULTS
SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2-related factor 2 signaling.
CONCLUSION
These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.
背景
人脐带间充质干细胞（hUCMSCs）已成为治疗免疫和炎症性疾病的有前途的方法。然而，如何在冷藏和运输过程中保持其活性和独特特性是影响 hUCMSCs 治疗效率的关键因素之一。五味子素有许多作为天然药物的细胞保护功能。在这项研究中，我们研究了五味子素 B（SchB）对 hUCMSCs 低温保存的保护作用。
方法
从华通氏胶中分离 hUCMSCs。然后，将 hUCMSCs 暴露于冷藏（4°C）和 24 小时复温。之后，评估细胞活力、表面标志物、免疫调节作用、活性氧（ROS）、线粒体完整性、凋亡相关和抗氧化蛋白表达水平。
结果
SchB 显著减轻了细胞损伤，并维持了 hUCMSCs 的独特特性，如分化潜能、表面标志物水平和免疫调节作用。SchB 对低温保存后 hUCMSCs 的保护作用似乎与其抑制凋亡和核因子红细胞 2 相关因子 2 信号介导的抗氧化应激作用有关。
结论
这些结果表明，SchB 可用作 hUCMSCs 低温保存的试剂。
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