São Leopoldo Mandic School of Dentistry, Dr. José Rocha Junqueira Street 13, Campinas, SP, Brazil.
University Center of Patos de Minas, School of Dentistry, Avenida Maraba 831, Patos de Minas, MG, Brazil.
Photodiagnosis Photodyn Ther. 2021 Mar;33:102182. doi: 10.1016/j.pdpdt.2021.102182. Epub 2021 Jan 8.
The aim of this study was to analyze, in vitro, the reduction of Enterococcus faecalis in root canals of primary teeth after final irrigation and photodynamic therapy (PDT) use. Twenty primary molars were contaminated with Enterococcus faecalis. The teeth were randomly distributed into four groups according to the irrigation solutions and PDT use: G1 (Saline solution and no PDT use), G2 (17 % EDTA and no PDT use), G3 (Saline solution and PDT use) and G4 (17 % EDTA and PDT use). For PDT, 0.005 % toluidine blue was chosen as the photosensitizer, which was inserted in the canals with sterile paper cones. Bacterial counts were performed with a BHI test in blood agar plate, where bacteria were collected inside the canal for 30 s using sterile paper cones. The collection took place before and after the irrigation and PDT protocols. The samples were diluted, spread onto a blood agar plate and then incubated at 37 °C for 24 h. There was a reduction of the microbiota from the irrigation solutions before and after the final irrigation for all groups. It was observed a statistically significant reduction (p < 0,05) when PDT was used (97.6 % at the saline solution and 89.8 % at the 17 % EDTA) when compared to the groups with no PDT use. Our data demonstrated that PDT, according to the parameters used, increased the disinfection performance of the solutions tested in the root canals of primary teeth.
本研究旨在分析终末冲洗和光动力疗法（PDT）使用后对乳牙根管内粪肠球菌的减少作用。将 20 颗乳磨牙污染粪肠球菌。根据冲洗液和 PDT 使用情况，将牙齿随机分为四组：G1（生理盐水，不使用 PDT）、G2（17% EDTA，不使用 PDT）、G3（生理盐水，使用 PDT）和 G4（17% EDTA 和 PDT 使用）。PDT 选用 0.005%甲苯胺蓝作为光敏剂，用无菌纸尖将其插入根管。采用 BHI 试验在血琼脂平板上进行细菌计数，使用无菌纸尖在 30 s 内从根管内采集细菌。在冲洗和 PDT 方案前后进行采样。将样品稀释，涂抹于血琼脂平板上，然后在 37°C 孵育 24 h。所有组的终末冲洗前后，冲洗液中的微生物群均减少。与未使用 PDT 的组相比，使用 PDT 时观察到统计学上显著减少（p < 0.05）（生理盐水组为 97.6%，17% EDTA 组为 89.8%）。我们的数据表明，根据所用参数，PDT 增加了测试溶液在乳牙根管中的消毒性能。
