Hammarback J A, McCarthy J B, Palm S L, Furcht L T, Letourneau P C
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.
Dev Biol. 1988 Mar;126(1):29-39. doi: 10.1016/0012-1606(88)90235-7.
Substrate-bound laminin pathways prepared by the method of Hammarback et al. [J.A. Hammarback, S.L. Palm, L.T. Furcht, and P.C. Letourneau (1985). J. Neurosci. Res. 13, 213-220] guided peripheral nervous system neurites (dissociated dorsal root ganglia and sympathetic ganglia) and central nervous system neurites (dissociated spinal cord and brain). Guidance of individual growth cones by 7- to 10-micron-wide laminin pathways was observed using time-lapse video microscopy. Fibronectin pathways, produced by the method used for laminin pathways, did not guide neurites. The guidance effect of laminin pathways was quantified and found to correlate with the concentration of laminin initially applied to the substratum. The concentration of laminin initially applied to the substratum also correlated with increased adhesivity of dorsal root ganglia (DRG) neurons to laminin constituting the pathways relative to uv-irradiated laminin that borders the pathways. The guidance effect of laminin pathways was blocked by anti-laminin antibodies or by laminin but not by anti-fibronectin antibodies. This study demonstrates that guidance of DRG neurites by laminin occurs at the growth cone in a manner consistent with the hypothesis of guidance by differential neuron-to-substratum adhesivity.
通过哈马巴克等人[J.A.哈马巴克、S.L.帕尔姆、L.T.弗奇特和P.C.勒图尔诺（1985年）。《神经科学研究杂志》13卷，213 - 220页]的方法制备的与底物结合的层粘连蛋白途径引导外周神经系统神经突（解离的背根神经节和交感神经节）以及中枢神经系统神经突（解离的脊髓和脑）。使用延时视频显微镜观察到7至10微米宽的层粘连蛋白途径对单个生长锥的引导作用。通过用于层粘连蛋白途径的方法产生的纤连蛋白途径不能引导神经突。对层粘连蛋白途径的引导作用进行了量化，发现其与最初施加到底物上的层粘连蛋白浓度相关。最初施加到底物上的层粘连蛋白浓度还与背根神经节（DRG）神经元相对于构成途径边界的紫外线照射层粘连蛋白对构成途径的层粘连蛋白的粘附性增加相关。层粘连蛋白途径的引导作用被抗层粘连蛋白抗体或层粘连蛋白阻断，但不被抗纤连蛋白抗体阻断。这项研究表明，层粘连蛋白对DRG神经突的引导作用发生在生长锥处，其方式与神经元 - 底物粘附性差异引导假说一致。
