Vöhringer Maria, Hartleb Wibke, Lienkamp Karen
Department of Microsystems Engineering (IMTEK) and Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), Albert-Ludwigs-Universität, Georges-Köhler-Allee 103, 79110 Freiburg, Germany.
ACS Biomater Sci Eng. 2017 Jun 12;3(6):909-921. doi: 10.1021/acsbiomaterials.7b00140. Epub 2017 May 2.
A manufacturing process for the site-selective modification of structured (bio)material surfaces with two different polymers/biomolecules is presented. In the first step, a chemical surface contrast is created (e.g., a gold-on-silicon contrast obtained by colloidal lithography), and is combined with two orthogonal surface reactions for polymer/biomolecule immobilization. To demonstrate this, an antimicrobial SMAMP polymer and a protein-repellent polyzwitterion were site-selectively surface-immobilized on the gold-silicon structures. By varying the structure spacing and the surface architecture, structure-property relationships for the interaction of these bifunctional polymer surfaces with bacteria and proteins were obtained (studied by fluorescence microscopy, atomic force microscopy, surface plasmon resonance spectroscopy, and antimicrobial assays). At 1 μm spacing, a fully antimicrobially active bifunctional material was obtained, which also near-quantitatively reduced protein adhesion. As the process is generally applicable to polymers/biomolecules with aliphatic CH-groups, it is an interesting platform technology for site-selectively functionalized bifunctional (Bio)MEMS.
本文介绍了一种用于对结构化（生物）材料表面进行位点选择性修饰的制造工艺，该工艺可使用两种不同的聚合物/生物分子。第一步，创建化学表面对比度（例如，通过胶体光刻获得的金 - 硅对比度），并将其与用于聚合物/生物分子固定的两种正交表面反应相结合。为了证明这一点，将抗菌SMAMP聚合物和蛋白质排斥性聚两性离子位点选择性地表面固定在金 - 硅结构上。通过改变结构间距和表面结构，获得了这些双功能聚合物表面与细菌和蛋白质相互作用的结构 - 性质关系（通过荧光显微镜、原子力显微镜、表面等离子体共振光谱和抗菌试验进行研究）。在1μm的间距下，获得了一种具有完全抗菌活性的双功能材料，该材料还几乎定量地减少了蛋白质粘附。由于该工艺通常适用于具有脂肪族CH基团的聚合物/生物分子，因此它是一种用于位点选择性功能化双功能（生物）微机电系统的有趣平台技术。
