Wu Yan, Gao Qing, Nie Jing, Fu Jian-Zhong, He Yong
State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China.
Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China.
ACS Biomater Sci Eng. 2017 Apr 10;3(4):601-607. doi: 10.1021/acsbiomaterials.7b00084. Epub 2017 Mar 8.
As a kind of facile tool, microfluidic paper-based analytical devices (μPADs) have been widely used in analytical and biomedical fields. However, because we lack the ability to control the continuous perfusion of these devices, they are not generally used in fields that require continuous flow, especially biofluidics fields such as cell culturing, drug screening, and organs on chips. In this study, we designed a novel, low-cost, and compact platform that can be used to control the continuous perfusion of μPADs. As most of the parts of this platform can be created using a three-dimensional (3D) desktop printer, our platform can be easily duplicated by other researchers. We demonstrate that with our system, μPADs can be promising paper-based biofluidic platforms for cell culturing and drug screening.
作为一种便捷的工具，基于微流控纸的分析装置（μPADs）已在分析和生物医学领域中得到广泛应用。然而，由于我们缺乏控制这些装置连续灌注的能力，它们通常不用于需要连续流动的领域，特别是诸如细胞培养、药物筛选和芯片器官等生物流体学领域。在本研究中，我们设计了一种新颖、低成本且紧凑的平台，可用于控制μPADs的连续灌注。由于该平台的大部分部件都可以使用三维（3D）桌面打印机制作，其他研究人员可以轻松复制我们的平台。我们证明，借助我们的系统，μPADs有望成为用于细胞培养和药物筛选的基于纸的生物流体平台。
