Li Xiangfeng, Wang Menglu, Deng Yanglong, Chen Xuening, Xiao Yumei, Zhang Xingdong
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
ACS Biomater Sci Eng. 2017 Aug 14;3(8):1557-1566. doi: 10.1021/acsbiomaterials.7b00232. Epub 2017 Jul 10.
Spherical calcium phosphate (Ca-P) granules show advantages in filling bone cavity defects. In this study, gelatinizing technology combined with microsphere-sintering and gas-foaming methods was applied to fabricate porous spherical Ca-P bioceramic granules. The obtained three kinds of Ca-P granules (i.e., hydroxyapatite, HA-G; biphasic calcium phosphate, BCP-G; and tricalcium phosphate, TCP-G) exhibited uniformly spherical shapes and interconnected pore structures with controlled macropores (about 600 μm), and abundant minor- (10-100 μm) to microsized (<10 μm) pores. The obtained Ca-P granules contained only the HA and β-TCP phases, but their phase ratios (HA/β-TCP) had some changes in the sintering process. All of the Ca-P granules were favorable for serum protein adsorption and bonelike apatite formation, and TCP-G was superior to the other two. Cell-culturing results showed that these Ca-P granules could promote cell adhesion, proliferation, and expression of a series of osteogenic genes. These findings demonstrated that these Ca-P granules had good bioactivity and pro-osteogenic ability, especially for BCP-G and TCP-G. Therefore, the porous spherical Ca-P bioceramic granules fabricated by the novel method show great potential to serve as good candidates for bone defect filling materials.
球形磷酸钙（Ca-P）颗粒在填充骨腔缺损方面具有优势。在本研究中，采用糊化技术结合微球烧结和气体发泡方法制备了多孔球形Ca-P生物陶瓷颗粒。所制备的三种Ca-P颗粒（即羟基磷灰石，HA-G；双相磷酸钙，BCP-G；以及磷酸三钙，TCP-G）呈现出均匀的球形形状以及相互连通的孔隙结构，具有可控的大孔（约600μm），以及丰富的从10 - 100μm的次级孔到小于10μm的微孔。所获得的Ca-P颗粒仅含有HA和β-TCP相，但它们的相比例（HA/β-TCP）在烧结过程中发生了一些变化。所有的Ca-P颗粒都有利于血清蛋白吸附和类骨磷灰石形成，并且TCP-G优于其他两种。细胞培养结果表明，这些Ca-P颗粒能够促进细胞黏附、增殖以及一系列成骨基因的表达。这些发现表明，这些Ca-P颗粒具有良好的生物活性和成骨促进能力，尤其是对于BCP-G和TCP-G。因此，通过该新方法制备的多孔球形Ca-P生物陶瓷颗粒作为骨缺损填充材料的良好候选者具有巨大潜力。
