Liu Leyi, Wu Jie, Lv Shiyu, Xu Duoling, Li Shujun, Hou Wentao, Wang Chao, Yu Dongsheng
Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
Mater Today Bio. 2023 Nov 25;23:100866. doi: 10.1016/j.mtbio.2023.100866. eCollection 2023 Dec.
The significance of the osteogenesis-angiogenesis relationship in the healing process of bone defects has been increasingly emphasized in recent academic research. Surface topography plays a crucial role in guiding cellular behaviors. Metal-organic framework (MOF) is an innovative biomaterial with nanoscale structural and topological features, enabling the modulation of scaffold physicochemical properties. This study involved the loading of varying quantities of UiO-66 nanocrystals onto alkali-heat treated 3D-printed titanium scaffolds, resulting in the formation of hierarchical micro/nano topography named UiO-66/AHTs. The physicochemical properties of these scaffolds were subsequently characterized. Furthermore, the impact of these scaffolds on the osteogenic potential of BMSCs, the angiogenic potential of HUVECs, and their intercellular communication were investigated. The findings of this study indicated that 1/2UiO-66/AHT outperformed other groups in terms of osteogenic and angiogenic induction, as well as in promoting intercellular crosstalk by enhancing paracrine effects. These results suggest a promising biomimetic hierarchical topography design that facilitates the coupling of osteogenesis and angiogenesis.
骨缺损愈合过程中骨生成与血管生成关系的重要性在最近的学术研究中得到了越来越多的强调。表面形貌在引导细胞行为方面起着至关重要的作用。金属有机框架（MOF）是一种具有纳米级结构和拓扑特征的创新生物材料，能够调节支架的物理化学性质。本研究将不同数量的UiO-66纳米晶体负载到碱热处理的3D打印钛支架上，形成了名为UiO-66/AHTs的分级微/纳米形貌。随后对这些支架的物理化学性质进行了表征。此外，还研究了这些支架对骨髓间充质干细胞（BMSCs）成骨潜能、人脐静脉内皮细胞（HUVECs）血管生成潜能及其细胞间通讯的影响。本研究结果表明，1/2UiO-66/AHT在成骨和血管生成诱导方面以及通过增强旁分泌作用促进细胞间串扰方面优于其他组。这些结果表明了一种有前景的仿生分级形貌设计，有助于骨生成与血管生成的耦合。
