Kim Dajung, Kim Seonmok, Jung Seunho
Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Konkuk University, Seoul 05029, Korea.
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Applications (UBITA), Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.
Polymers (Basel). 2021 Jan 8;13(2):202. doi: 10.3390/polym13020202.
In the present study, a polysaccharide metallohydrogel was successfully fabricated using succinoglycan and trivalent chromium and was verified via Fourier transform infrared spectroscopy, differential scanning calorimetry analysis, thermogravimetric analysis (TGA), field emission scanning electron microscopy, and rheological measurements. Thermal behavior analysis via TGA indicated that the final mass loss of pure succinoglycan was 87.8% although it was reduced to 65.8% by forming a hydrogel with trivalent chromium cations. Moreover, succinoglycan-based metallohydrogels exhibited improved mechanical properties based on the added concentration of Cr and displayed a 10 times higher compressive stress and enhanced storage modulus (G') of 230% at the same strain. In addition, the pore size of the obtained SCx could be adjusted by changing the concentration of Cr. Gelation can also be adjusted based on the initial pH of the metallohydrogel formulation. This was attributed to crosslinking between chromium trivalent ions and hydroxyl/carboxyl groups of succinoglycan, each of which exhibits a specific pH-dependent behavior in aqueous solutions. It could be used as a soft sensor to detect Cr in certain biological systems, or as a soft matrix for bioseparation that allows control of pore size and mechanical strength by tuning the Cr concentration.
在本研究中，使用琥珀聚糖和三价铬成功制备了一种多糖金属水凝胶，并通过傅里叶变换红外光谱、差示扫描量热分析、热重分析（TGA）、场发射扫描电子显微镜和流变学测量进行了验证。通过TGA进行的热行为分析表明，纯琥珀聚糖的最终质量损失为87.8%，尽管与三价铬阳离子形成水凝胶后降至65.8%。此外，基于琥珀聚糖的金属水凝胶根据添加的Cr浓度表现出改善的机械性能，在相同应变下显示出高10倍的压缩应力和230%的增强储能模量（G'）。此外，通过改变Cr的浓度可以调节所得SCx的孔径。凝胶化也可以根据金属水凝胶配方的初始pH值进行调节。这归因于三价铬离子与琥珀聚糖的羟基/羧基之间的交联，它们在水溶液中各自表现出特定的pH依赖性行为。它可以用作检测某些生物系统中Cr的软传感器，或用作生物分离的软基质，通过调节Cr浓度来控制孔径和机械强度。
