Wang Yichong, Yu Sijiong, Li Yang, Zhang Shuang, Qi Xiaolong, Guo Kaijun, Guo Yong, Fortina Riccardo
College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Paolo Braccini 2, Grugliasco, TO 10095, Italy.
Animals (Basel). 2021 Jan 7;11(1):108. doi: 10.3390/ani11010108.
Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%-0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH-N), and methane (CH) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH-N ( < 0.05) compared to control. The 0.1%-0.4% PICB significantly decreased acetic acid content ( < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content ( < 0.05) and reduced the acetic acid/propionic acid ratio, CH content, and yield ( < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned ( < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters ( < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.
营养策略可用于减少反刍动物的温室气体排放。本文研究了从白苞蒿总苞中提取的多酚（PICB）对体外瘤胃发酵的影响。选用3头装有永久性瘤胃瘘管的健康安格斯公牛（350±50千克）作为瘤胃液供体。基础日粮添加5个剂量的PICB（0%-0.5%干物质（DM）），每个剂量重复3次。体外发酵24小时后测定挥发性脂肪酸（VFA）、氨氮浓度（NH-N）和甲烷（CH）产量，并监测96小时的产气量。试验进行了3次。结果表明，与对照组相比，添加PICB显著降低了NH-N（P<0.05）。0.1%-0.4%的PICB显著降低了乙酸含量（P<0.05）。添加0.2%和0.3%的PICB显著提高了丙酸含量（P<0.05），并降低了乙酸/丙酸比例、CH含量和产量（P<0.05）。上述所有参数均呈现极显著的二次响应，随着PICB水平的增加（P<0.01）。PICB浓度的增加导致96小时动态发酵参数呈现极显著的线性和二次响应（P<0.01）。我们的结果表明，0.2%的PICB对体外瘤胃发酵效率的影响最佳，并减少了温室气体的产生。
