Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania 'Luigi Vanvitelli', Via Vivaldi 43, 81100 Caserta, Italy.
Pharmazeutisches Institut, Abteilung Pharmazeutische Biologie, Christian-Albrechts-Universität zu Kiel, Gutenbergstraße 76, 24118 Kiel, Germany.
Molecules. 2022 Mar 29;27(7):2217. doi: 10.3390/molecules27072217.
In recent years, animal husbandry has aimed at improving the conditions of livestock animals useful for humans to solve environmental and health problems. The formulation of animal feeds or supplements based on antioxidant plant compounds is considered a valuable approach and an alternative for livestock productivity. Forest biomass materials are an underestimated source of polyphenolic compounds whose sustainable recovery could provide direct benefits to animals and, indirectly, human nutrition. In this context, an alcohol extract from leaves of L. was first investigated through an untargeted ultra-high-performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) approach. Then, it was fractionated into a fatty acid-rich and a polyphenolic fraction, as evidenced by total lipid, phenol, and flavonoid content assays, with antiradical and reducing activity positively correlated to the latter. When tested in vitro with rumen liquor to evaluate changes in the fermentative parameters, a significant detrimental effect was exerted by the lipid-rich fraction, whereas the flavonoid-rich one positively modulated the production of volatile fatty acids (i.e., acetate, butyrate, propionate, etc.).
近年来，畜牧业旨在改善对人类有用的牲畜的条件，以解决环境和健康问题。基于抗氧化植物化合物的动物饲料或补充剂的配方被认为是一种有价值的方法，也是提高畜牧业生产力的一种替代方法。森林生物质材料是多酚化合物的一个被低估的来源，其可持续回收可以为动物提供直接的好处，并间接地为人类营养提供好处。在这种情况下，首次通过非靶向超高效液相色谱-高分辨率串联质谱（UHPLC-HRMS/MS）方法研究了 L. 的叶醇提物。然后，它被分为富含脂肪酸和富含多酚的两个部分，这可以通过总脂质、酚和类黄酮含量的测定来证明，自由基清除活性和还原活性与后者呈正相关。当用瘤胃液进行体外测试以评估发酵参数的变化时，富含脂质的部分会产生显著的有害影响，而富含类黄酮的部分则会积极调节挥发性脂肪酸（即乙酸盐、丁酸盐、丙酸盐等）的产生。
