Fornabaio D M, Alterman A L, Stackpole C W
Department of Pathology, New York Medical College, Valhalla.
Invasion Metastasis. 1988;8(1):1-16.
Spontaneous metastasis from tumor transplants of two representative mouse B16 melanoma clones, G3.5 and G3.12, was examined experimentally to determine whether initial dissemination to the lungs, or secondary systemic spread from established lung metastases, resulted from organ-specific tropism or from nonspecific trapping of circulating tumor cells in capillary beds. In parabiosed mice, subcutaneous tumors metastasized extensively within hosts, but guests remained metastasis-free except following the rare involvement of the parabiotic junction during secondary spread. Intrasplenic tumor transplants metastasized to the liver, whereas intrarenal transplants metastasized to the lungs, reflecting patterns of venous drainage. Subcutaneous implants of neonatal lung and kidney in the flank opposite from the site of tumor initiation acquired metastases only during secondary systemic spread, and there was no evidence of organ selectivity. Metastases from various organs, and derived cell lines, when transplanted subcutaneously grew into tumors that initially metastasized exclusively to the lungs. These results indicate that both initial and secondary metastases of these B16 melanoma transplants occurred by nonspecific trapping of tumor cells in the first capillary bed encountered. In contrast, organ colonization following intravenous injection of tumor cells frequently proceeded beyond the first capillary bed.
对两个具有代表性的小鼠B16黑色素瘤克隆G3.5和G3.12的肿瘤移植进行了自发转移的实验研究，以确定肿瘤最初向肺部的扩散，或已形成的肺转移灶的继发性全身扩散，是由器官特异性嗜性引起，还是由循环肿瘤细胞在毛细血管床中的非特异性滞留所致。在联体小鼠中，皮下肿瘤在宿主体内广泛转移，但受体小鼠除了在继发性扩散过程中罕见地累及联体连接部位外，仍无转移。脾内肿瘤移植转移至肝脏，而肾内移植转移至肺部，反映了静脉引流模式。在肿瘤起始部位对侧的胁腹皮下植入新生肺和肾，仅在继发性全身扩散期间出现转移，且没有器官选择性的证据。来自各种器官和衍生细胞系的转移瘤，皮下移植后长成肿瘤，最初仅转移至肺部。这些结果表明，这些B16黑色素瘤移植的原发性和继发性转移均是由于肿瘤细胞在遇到的第一个毛细血管床中被非特异性滞留所致。相比之下，静脉注射肿瘤细胞后的器官定植常常会超出第一个毛细血管床。
