Hart I R, Fidler I J
Cancer Res. 1980 Jul;40(7):2281-7.
The preferential growth of B16 melanoma metastases in specific organs was studied. Following the i.v. injection of B16 melanoma cells into syngeneic C57BL/6 mice, tumor growths developed in the in situ lungs and in grafts of pulmonary or ovarian tissue implanted either s.c. or i.m. In contrast, neoplastic lesions failed to develop in control grafts of similarly implanted renal tissue or at the site of a surgical trauma. Parabiosis experiments suggested that the growth of the B16 melanoma in ectopic lung or ovary tissue was due to the immediate arrest of circulating neoplastic cells and not to shedding of malignant cells from foci growing in the in situ lungs. Quantitative analysis of tumor cell arrest and distribution using cells labeled with [125I]-5-iodo-2'-deoxyuridine indicated that the growth of tumors in the implanted organs was not due to an enhanced initial arrest of B16 cells. No significant differences in immediate tumor cell arrest were detected between implanted fragments of lungs (tumor positive) and kidney (tumor negative) or between organ-bearing and contralateral control limbs. We conclude that the outcome of metastasis is dependent on both tumor cell properties and host factors. This conclusion supports the "seed and soil" hypothesis to explain the nonrandom pattern of cancer metastasis.
研究了B16黑色素瘤转移灶在特定器官中的优先生长情况。将B16黑色素瘤细胞经静脉注射到同基因C57BL/6小鼠体内后，原位肺以及皮下或肌肉内植入的肺或卵巢组织移植物中出现了肿瘤生长。相比之下，同样植入的肾组织对照移植物或手术创伤部位未出现肿瘤性病变。联体共生实验表明，B16黑色素瘤在异位肺或卵巢组织中的生长是由于循环肿瘤细胞的立即停滞，而非原位肺中生长灶的恶性细胞脱落。使用[125I]-5-碘-2'-脱氧尿苷标记的细胞对肿瘤细胞停滞和分布进行定量分析表明，植入器官中肿瘤的生长并非由于B16细胞初始停滞增强所致。在肺（肿瘤阳性）和肾（肿瘤阴性）的植入片段之间，或在携带器官的肢体与对侧对照肢体之间，未检测到肿瘤细胞立即停滞的显著差异。我们得出结论，转移的结果取决于肿瘤细胞特性和宿主因素。这一结论支持“种子与土壤”假说来解释癌症转移的非随机模式。
