Sedaghat Bahareh, Hajjaran Homa, Sadjjadi Fatemeh Sadat, Heidari Soudabeh, Sadjjadi Seyed Mahmoud
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
BMC Res Notes. 2021 Jan 11;14(1):22. doi: 10.1186/s13104-020-05433-3.
Proper characterization of hydatid cyst fluid (HCF) is useful for diagnostic and follow up purposes of cystic echinococcosis/hydatidosis, which is an important zoonotic disease. In this regard, proteomics methods are very helpful. The present study was conducted to compare three protein extraction methods for HCF collected from sheep liver hydatid cysts including, trichloracetic acid (TCA)/Acetone precipitation, TCA/Acetone along with dialysis, and combination of 2-D Clean-up Kit and dialysis followed by two-dimensional electrophoresis (2-DE), to achieve better resolution in the proteomic characterization of HCF proteins.
The 2-DE of TCA/Acetone products showed a lot of smears in the background of gels; TCA/Acetone with dialysis showed greatly reduced smears while the 2-D Clean-up Kit together with dialysis showed sharp spots and least smears. Three-dimensional images of separated spots created by Progenesis SameSpots software showed the best result was achieved by 2-D Clean-up Kit and dialysis.
恰当地鉴定棘球蚴囊液（HCF）对于囊型包虫病/包虫病（一种重要的人畜共患病）的诊断及随访具有重要意义。在此方面，蛋白质组学方法非常有用。本研究旨在比较从绵羊肝棘球蚴囊中采集的HCF的三种蛋白质提取方法，包括三氯乙酸（TCA）/丙酮沉淀法、TCA/丙酮结合透析法，以及二维净化试剂盒结合透析法，之后进行二维电泳（2-DE），以在HCF蛋白质的蛋白质组学鉴定中获得更好的分辨率。
TCA/丙酮产物的二维电泳在凝胶背景上显示出大量拖尾；TCA/丙酮结合透析法显示拖尾大大减少，而二维净化试剂盒结合透析法显示出清晰的斑点且拖尾最少。由Progenesis SameSpots软件生成的分离斑点的三维图像显示，二维净化试剂盒结合透析法取得了最佳结果。
