细粒棘球蚴（G1-G3）和加拿大棘球蚴（G6/G7）原头节同工酶模式的比较。
Comparison of Isoenzyme Pattern of Echinococcus granulosus sensu stricto (G1-G3) and E. canadensis (G6/G7) Protoscoleces.
机构信息
Students Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Iran Biomed J. 2023 Mar 1;27(2 & 3):136-45. doi: 10.61186/ibj.3815.
BACKGROUND
Different genotypes of Echinococcus granulosus sensu lato (s.l.) infect humans and ungulate animals, causing cystic echinococcosis. Simultaneous isoenzyme, as well as molecular characterizations of this parasite, has not yet been investigated in Iran. The present study aimed to evaluate the isoenzyme pattern of the E. granulosus sensu stricto (s.s.) and E. canadensis genotypes in Iran.
METHODS
A total of 32 (8 humans and 24 animals) cystic echinococcosis cysts were isolated from Shiraz, Tehran, Ilam, and Birjand from May 2018 to December 2020. The DNAs were extracted and their genotypes were determined by molecular methods. Enzymes were extracted from the cysts and subjected to polyacrylamide gel electrophoresis. The activities of glucose-6-phosphate sehydrogenase (G6PD), malate dehydrogenase (MDH), malic enzyme (ME), nucleoside hydrolyse 1 (NH1), and isocitrate dehydrogenase (ICD) were examined in the cyst samples using isoenzyme method and compared it with the genotyping findings.
RESULTS
DNA sequence analysis of the samples showed that the specimens contained 75% E. granulosus s.s. (G1) and 25% E. canadensis (G6) genotypes. The isoenzyme pattern of ICD in both genotypes produced a six-band pattern with different relative factors. The G6PD also produced two bands with different relative migrations in both genotypes. The MDH and NH1 systems revealed a two-band pattern, while only one band was generated in the ME enzyme in the E. granulosus s.s. genotype. In the E. canadensis, the MDH and NH1 enzymes showed one band, and the ME enzyme represented a two-band pattern.
CONCLUSION
Our findings suggest that E. granulosus s.s. and E. canadensis genotypes have entirely different isoenzyme patterns for NH1, G6PD, MDH, and ME.
背景
不同基因型的细粒棘球绦虫（Echinococcus granulosus sensu lato，s.l.）感染人类和有蹄类动物，引起包虫病。该寄生虫的同工酶以及分子特征在伊朗尚未得到研究。本研究旨在评估伊朗细粒棘球蚴（E. granulosus sensu stricto，s.s.）和加拿大棘球蚴基因型的同工酶模式。
方法
从 2018 年 5 月至 2020 年 12 月，从设拉子、德黑兰、伊拉姆和比尔詹德共分离出 32 例（8 例人类和 24 例动物）包虫病囊肿。从囊肿中提取 DNA，并用分子方法确定其基因型。从囊肿中提取酶，并用聚丙烯酰胺凝胶电泳进行同工酶分析。采用同工酶法检测囊样中葡萄糖-6-磷酸脱氢酶（G6PD）、苹果酸脱氢酶（MDH）、苹果酸酶（ME）、核苷水解酶 1（NH1）和异柠檬酸脱氢酶（ICD）的活性，并与基因分型结果进行比较。
结果
样本的 DNA 序列分析显示，标本中含有 75%的细粒棘球蚴 s.s.（G1）和 25%的加拿大棘球蚴（G6）基因型。两种基因型的 ICD 同工酶图谱均产生具有不同相对因子的六带图谱。两种基因型的 G6PD 也产生具有不同相对迁移的两条带。MDH 和 NH1 系统呈现出两条带模式，而 ME 酶在细粒棘球蚴 s.s.基因型中仅产生一条带。在加拿大棘球蚴中，MDH 和 NH1 酶显示一条带，ME 酶显示两条带模式。
结论
我们的研究结果表明，细粒棘球蚴 s.s.和加拿大棘球蚴基因型的 NH1、G6PD、MDH 和 ME 同工酶模式完全不同。
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