支原体关节炎犊牛滑液中炎性细胞因子mRNA和蛋白质水平
Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves.
作者信息
Nishi Koji, Gondaira Satoshi, Okamoto Mariko, Matsuda Kazuya, Sato Ayano, Kato Toshihide, Sasagawa Misawo, Tanaka Takahiro, Higuchi Hidetoshi
机构信息
Animal Health Laboratory, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Present address: Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
出版信息
J Vet Med Sci. 2021 Jan 14;83(1):31-35. doi: 10.1292/jvms.20-0491. Epub 2021 Jan 8.
Bovine Mycoplasma arthritis (MA) is caused by Mycoplasma bovis and exhibits severe clinical symptoms. However, the pathophysiology of bovine MA is incompletely understood. In this study, we examined the cytokine mRNA expression of synovial fluid (SF) cells and cytokine concentrations in the SF of MA calves. The SF was isolated from five clinically healthy (control) and seven MA calves. mRNA and protein levels of interleukin (IL)-1β, IL-6, IL-8, IL-12, and IL-17 in the SF from MA calves were significantly higher than those from control calves. Our results indicate that SF cells produce inflammatory cytokines, which mainly contribute to the severe inflammatory response in the joints of the MA calves.
牛支原体关节炎（MA）由牛支原体引起，具有严重的临床症状。然而，牛MA的病理生理学尚未完全明确。在本研究中，我们检测了MA犊牛滑液（SF）细胞的细胞因子mRNA表达及SF中的细胞因子浓度。从5头临床健康（对照）犊牛和7头MA犊牛中分离出SF。MA犊牛SF中白细胞介素（IL）-1β、IL-6、IL-8、IL-12和IL-17的mRNA和蛋白质水平显著高于对照犊牛。我们的结果表明，SF细胞产生炎性细胞因子，这主要导致了MA犊牛关节中的严重炎症反应。
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