Mahmood Fazal, Khan Ahrar, Hussain Riaz, Khan Imtiaz Ahmad, Abbas Rao Zahid, Ali Hafiz Muhammad, Younus Muhammad
Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38040, Pakistan.
University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur 63100, Pakistan.
Microb Pathog. 2017 Jun;107:404-408. doi: 10.1016/j.micpath.2017.04.003. Epub 2017 Apr 4.
Mycoplasma bovis (M. bovis) is an important bacterium, causing severe respiratory infection, and arthritis in dairy animals worldwide. This study is based on 50 suckling calves among which 15 showed respiratory distress, lameness and swollen joints and died later. M. bovis was isolated and identified from all dead (n = 15) and live (17.14%; 06 out of 35) calves on the basis of bacteriological examination. In morbid calves, the carpus and stifle joints were severely affected, while necropsy revealed multiple well-circumscribed calcified abscesses and caseous exudates in cranio-ventral and diaphragmatic lobes of lungs. Suppurative polyarthritis, fibrino-suppurative, teno-synovitis and otitis media were the common and striking lesion observed at postmortem examination. Histopathological examination revealed broncho-interstitial pneumonia and necrotic fibrino-purulent broncho-pneumonia in lungs. Similarly, synovial membranes and joints revealed presence of multiple foci of liquefactive necrosis surrounded by lymphocytes, plasma cells, macrophages and peripheral fibroplasia. In the bacteriological investigations, the characteristic fried egg colonies of M. bovis further confirmed this infection in all suspected cases. In conclusion, the current clinico-histo-pathological features are the depictive picture, and is the first report of M. bovis infection in calves in Pakistan.
牛支原体是一种重要的细菌，可在全球范围内引起奶牛严重的呼吸道感染和关节炎。本研究基于50头哺乳犊牛展开，其中15头出现呼吸窘迫、跛行和关节肿胀，随后死亡。基于细菌学检查，从所有死亡犊牛（n = 15）和存活犊牛（17.14%；35头中的6头）中分离并鉴定出牛支原体。在患病犊牛中，腕关节和膝关节受到严重影响，而尸检显示肺的颅腹叶和膈叶有多个边界清晰的钙化脓肿和干酪样渗出物。化脓性多关节炎、纤维蛋白化脓性腱鞘炎和中耳炎是死后检查中常见且显著的病变。组织病理学检查显示肺中有支气管间质性肺炎和坏死性纤维蛋白化脓性支气管肺炎。同样，滑膜和关节显示存在多个液化性坏死灶，周围有淋巴细胞、浆细胞、巨噬细胞和外周纤维组织增生。在细菌学调查中，牛支原体典型的荷包蛋菌落进一步证实了所有疑似病例的感染情况。总之，当前的临床组织病理学特征是具有代表性的，并且是巴基斯坦犊牛感染牛支原体的首次报告。
