Molecular Biology Laboratory, Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.
Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei, Taiwan.
Nutr Cancer. 2022;74(1):225-236. doi: 10.1080/01635581.2020.1870702. Epub 2021 Jan 12.
Liver cancer is the most common cause of mortality in men and women. The aim of this study was to evaluate the methanolic extract of aerial parts of L (LS) for its anticancer activity on HCC cell lines. Human HCC cell lines, Huh-7 and HSC-T6 were used for cytotoxicity assay, determination of ROS and gene expression analysis (p53, Bcl-2, Bax, caspase-3, caspase-8, and caspase-9). Western blotting was used to assess ERK, AKT, and Caspase-3 activation. HPLC-MS analysis was also performed to determine the phytochemicals present in LS-M extract. LS-M extract has increased the expression of proapoptotic genes, including , , and and down-regulated the activation of ERK and Akt. The caspase-3 activity as well as the ROS generation were significantly increased in a dose-dependent manner compared to control. The carotenoids, lutein and beta carotene present in LS-M extract exhibited anticancer activity. In overall, the methanolic extract of LS induces apoptosis in Huh-7 as well as in HSC-T6 cells possibly involving a ROS-mediated mitochondrial signaling pathway. The methanolic extract of LS induces apoptosis in Huh-7 as well as in HSC-T6 cells possibly involving a ROS-mediated mitochondrial signaling pathway[Formula: see text].
肝癌是男性和女性死亡的最常见原因。本研究旨在评估 L 的地上部分的甲醇提取物对 HCC 细胞系的抗癌活性。人肝癌细胞系 Huh-7 和 HSC-T6 用于细胞毒性测定、ROS 测定和基因表达分析（p53、Bcl-2、Bax、caspase-3、caspase-8 和 caspase-9）。Western blotting 用于评估 ERK、AKT 和 Caspase-3 的激活。还进行了 HPLC-MS 分析以确定 LS-M 提取物中存在的植物化学物质。LS-M 提取物增加了促凋亡基因的表达，包括 、 、 和 ，并下调了 ERK 和 Akt 的激活。与对照组相比，半胱天冬酶-3 活性以及 ROS 的产生呈剂量依赖性显著增加。LS-M 提取物中存在的类胡萝卜素叶黄素和β-胡萝卜素表现出抗癌活性。总的来说，LS 的甲醇提取物可能通过 ROS 介导的线粒体信号通路诱导 Huh-7 以及 HSC-T6 细胞凋亡。
