Phillis J W, O'Regan M H, Walter G A
Department of Physiology, Wayne State University, School of Medicine, Detroit 48201.
J Cereb Blood Flow Metab. 1988 Apr;8(2):179-85. doi: 10.1038/jcbfm.1988.47.
The cerebral cortical cup technique has been used to study the effects of nifedipine and felodipine on adenosine and inosine release from the rat brain. After basal and hypoxia (8% 02)-evoked control levels of purine release had been established, these 1,4-dihydropyridine calcium antagonists were administered intraperitoneally (1 mg/kg). Both agents depressed basal levels of purine efflux and suppressed the hypoxia-evoked release of adenosine and inosine. An inhibition of the transporter that mediates purine efflux from brain cells is likely to account for the suppression of release from the cerebral cortex. A reduced release of adenosine into the interstitial space also explains the ability of both agents to block the increase in CBF evoked by hypoxic challenges.
大脑皮质杯技术已被用于研究硝苯地平和非洛地平对大鼠脑内腺苷和肌苷释放的影响。在确定嘌呤释放的基础水平和缺氧（8%氧气）诱发的对照水平后，将这些1,4 - 二氢吡啶类钙拮抗剂腹腔注射（1毫克/千克）。两种药物均降低了嘌呤流出的基础水平，并抑制了缺氧诱发的腺苷和肌苷释放。介导嘌呤从脑细胞流出的转运体受到抑制可能是大脑皮质释放受抑制的原因。腺苷向细胞间隙释放的减少也解释了两种药物阻断缺氧刺激引起的脑血流量增加的能力。
