Phillis J W, Walter G A, O'Regan M H, Stair R E
Department of Physiology, Wayne State University, School of Medicine, Detroit, Michigan 48201.
J Cereb Blood Flow Metab. 1987 Dec;7(6):679-86. doi: 10.1038/jcbfm.1987.122.
The cerebral cortical cup technique was used to monitor changes in adenosine and inosine levels in the rat cerebral cortex during periods of hypoxia, anoxia, or hemorrhagic hypotension. Basal levels of adenosine and inosine in cortical perfusates stabilized within 10 min at concentrations of 30-50 and 75-130 nM, respectively. Comparable levels were observed in normal CSF collected from the cisterna magna. Reductions in the oxygen content of the inspired air (14, 12, 8, and 5% oxygen) resulted in increases in the adenosine and inosine levels in the cortical perfusates, the magnitude of the increase being progressively more pronounced with greater reductions in the oxygen content. Cerebral anoxia/ischemia, induced by 100% nitrogen inhalation, caused a rapid increase in the adenosine and inosine contents of the cortical perfusates. Hemorrhagic hypotension (46.1 +/- 1.7 mm Hg) of 5 min duration did not result in an elevated adenosine or inosine release. The results suggest that interstitial fluid adenosine levels are likely to be in the low nM range in the normoxic animal and are capable of rapid increases during hypoxic or anoxic episodes. The findings support the adenosine hypothesis of CBF regulation.
采用大脑皮质杯技术监测大鼠大脑皮质在缺氧、无氧或出血性低血压期间腺苷和肌苷水平的变化。皮质灌流液中腺苷和肌苷的基础水平在10分钟内分别稳定在30 - 50 nM和75 - 130 nM的浓度。从大脑大池收集的正常脑脊液中观察到类似水平。吸入空气中氧含量的降低（14%、12%、8%和5%氧气）导致皮质灌流液中腺苷和肌苷水平升高，随着氧含量降低幅度越大，升高幅度越明显。吸入100%氮气诱导的大脑缺氧/缺血导致皮质灌流液中腺苷和肌苷含量迅速增加。持续5分钟的出血性低血压（46.1±1.7 mmHg）并未导致腺苷或肌苷释放增加。结果表明，在常氧动物中，组织间液腺苷水平可能处于低纳摩尔范围，并且在缺氧或无氧发作期间能够迅速升高。这些发现支持了脑血流量调节的腺苷假说。
