Shabondama Soap Co, Ltd, Kitakyushu, Japan.
Department of Life and Environment Engineering, The University of Kitakyushu, Kitakyushu, Japan.
Int Wound J. 2021 Aug;18(4):467-477. doi: 10.1111/iwj.13547. Epub 2021 Jan 12.
Effective cleaning of a wound promotes wound healing and favours wound care as it can prevent and control biofilms. The presence of biofilm is associated with prolonged wound healing, increased wound propensity to infection, and delayed wound closure. Anionic potassium salts of fatty acids are tested with commonly used anionic surfactants, such as sodium laureth sulphate (SLES) and sodium lauryl sulphate/sodium dodecyl sulphate (SLS/SDS). The normal human dermal cells demonstrated significantly greater viability in fatty acid potassium, including caprylic acid (C8), capric acid (C10), lauric acid (C12), oleic acid (C18:1), and linoleic acid (C18:2), than in SLES or SLS after a 24-hour incubation. Cytotoxicity by LDH assay in a 5-minute culture in fatty acid potassium was significantly lower than in SLES or SLS. in vitro wound healing of human epidermal keratinocytes during the scratch assay in 24-hour culture was more significantly improved by fatty acid treatment than by SLES or SLS/SDS. In a live/dead assay of human epidermal keratinocytes, C8K and C18:1K demonstrated only green fluorescence, indicating live cells, whereas synthetic surfactants, SLES and SLS, demonstrated red fluorescence on staining with propidium iodide, indicating dead cells after SLES and SLS/SDS treatment. Potassium salts of fatty acids are useful wound cleaning detergents that do not interfere with wound healing, as observed in the scratch assay using human epidermal keratinocytes. As potassium salts of fatty acids are major components of natural soap, which are produced by natural oil and caustic potash using a saponification method, this may be clinically important in wound and peri-wound skin cleaning. In human chronic wounds, natural soap containing fatty acid potassium increased tissue blood flow based on laser speckle flowgraphs after 2 weeks (P < .05), in addition to removing the eschars and debris. Wound cleansing by natural soap of fatty acid potassium is beneficial for wound healing.
有效的伤口清洁可促进伤口愈合，并有利于伤口护理，因为它可以预防和控制生物膜。生物膜的存在与延长的伤口愈合、增加的伤口感染倾向和延迟的伤口闭合有关。脂肪酸的阴离子钾盐与常用的阴离子表面活性剂（如月桂醇聚醚硫酸酯钠（SLES）和月桂醇硫酸酯钠/十二烷基硫酸钠（SLS/SDS））一起进行测试。正常的人真皮细胞在脂肪酸钾中显示出明显更高的活力，包括辛酸（C8）、癸酸（C10）、月桂酸（C12）、油酸（C18:1）和亚油酸（C18:2），而在 24 小时孵育后，在 SLES 或 SLS 中则没有。在脂肪酸钾中进行 5 分钟的培养，用 LDH 测定细胞毒性明显低于 SLES 或 SLS。在划痕试验中，24 小时培养的人表皮角质形成细胞的体外伤口愈合经脂肪酸处理后明显优于 SLES 或 SLS/SDS。在人表皮角质形成细胞的活/死测定中，C8K 和 C18:1K 仅显示绿色荧光，表明活细胞，而合成表面活性剂 SLES 和 SLS 在与碘化丙啶染色后显示红色荧光，表明 SLES 和 SLS/SDS 处理后的死细胞。脂肪酸的钾盐是有用的伤口清洁洗涤剂，不会干扰伤口愈合，如用人表皮角质形成细胞进行划痕试验所观察到的那样。由于脂肪酸的钾盐是天然肥皂的主要成分，天然肥皂是通过皂化法由天然油和苛性钾制成的，这在伤口和伤口周围皮肤的清洁中可能具有重要的临床意义。在人类慢性伤口中，含脂肪酸钾的天然肥皂在 2 周后（P<.05）基于激光散斑血流图增加了组织血流，除了去除结痂和碎屑。脂肪酸钾的天然肥皂的伤口清洁有利于伤口愈合。
