Department of Medical Laboratory Science, Faculty of Health Sciences & Technology, Nnamdi Azikiwe University, Awka, Nigeria.
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Nnamdi Azikiwe University, Awka, Nigeria.
J Cosmet Dermatol. 2021 Sep;20(9):2989-2998. doi: 10.1111/jocd.13949. Epub 2021 Jan 21.
Clinical data exist that support the utility of topical probiotics for certain dermatological diseases such as atopic dermatitis, acne, and psoriasis. However, there is paucity of data on the use of live lactobacilli to control axillary malodor. The objective of this study was to determine whether application of topical oil-based cream containing live Lactobacilli could decrease malodor-producing bacteria in the axilla of healthy subjects.
To determine the effects of topical cream with live lactobacilli on malodor-producing bacteria in the axilla of healthy subjects.
PATIENTS/METHODS: Twenty-five adult volunteers comprising 12 males and 13 females provided informed consent. Axillary skin swabs were collected before and after 14 days application of topical cream containing live Lactobacillus pentosus KCA1. Bacterial DNA was extracted, and V4 region of the 16S rRNA was amplified and sequenced in a pair-end configuration on the Illumina MiSeq platform rendering 2 × 150 bp sequences. Microbial taxonomy to species level was generated using the Greengenes database. Linear discriminant analysis (LDA) effect size (LEfSe) was used to identify biologically and statistically significant differences in relative abundance.
Actinobacteria decreased from 70% to 24%, and Firmicutes increased from 26.6% to 73.9% among the female participants. In males, Actinobacteria decreased from 65% to 38%, while Firmicutes increased from 24% to 57%. Corynebacterium decreased from 62.91% to 36.63%, while Lactobacillus increased from 0.06% to 23.11%. In males, unliked females, there were reduction of Staphylococcus species associated with malodor, notably Staphylococcus hominis, Staphylococcus hemolyticus, and Staphylococcus lugdunensis. Bacterial functional gene- Pyridoxal protein dependent enzymes involved in biotransformation of malodor precursor to volatile thioalcohols were down-regulated.
Application of Lactobacillus pentosus KCA1 cream led to a significant decrease in the relative abundance of odor-producing Corynebacterium species in both female and male subjects. Some species associated with malodor especially Corynebacterium striatum, Corynebacterium jeikeium, Corynebacterium tuberculostearicum, and Staphylococcus hominis decreased by 96%, 73%, 7%, and 20.8%, respectively, in males.
临床数据表明，局部益生菌可用于治疗某些皮肤病，如特应性皮炎、痤疮和银屑病。然而，关于使用活菌乳杆菌控制腋窝异味的数据很少。本研究旨在确定局部含活菌乳杆菌的油基乳膏是否能减少健康受试者腋窝中产臭细菌。
确定局部乳杆菌活菌乳膏对健康受试者腋窝中产臭细菌的影响。
患者/方法：25 名成年志愿者（包括 12 名男性和 13 名女性）同意参与。在应用含有活菌戊糖片球菌 KCA1 的乳霜 14 天后，收集腋窝皮肤拭子。提取细菌 DNA，使用 Illumina MiSeq 平台进行 V4 区 16S rRNA 扩增和双端测序，生成 2×150bp 序列。使用 Greengenes 数据库生成微生物分类到种水平。线性判别分析（LDA）效应大小（LEfSe）用于识别相对丰度的生物学和统计学显著差异。
女性参与者中，放线菌从 70%减少到 24%，厚壁菌门从 26.6%增加到 73.9%。在男性中，放线菌从 65%减少到 38%，厚壁菌门从 24%增加到 57%。棒状杆菌减少从 62.91%到 36.63%，而乳杆菌从 0.06%增加到 23.11%。与女性不同，与异味相关的金黄色葡萄球菌种类减少，特别是人葡萄球菌、溶血葡萄球菌和路邓葡萄球菌。参与将异味前体转化为挥发性硫代醇的细菌功能基因-吡哆醛蛋白依赖酶被下调。
应用戊糖片球菌 KCA1 乳膏可显著降低女性和男性受试者中产臭棒状杆菌属物种的相对丰度。一些与异味相关的物种，特别是棒状杆菌属、棒状杆菌属、棒状杆菌属和人葡萄球菌，分别减少了 96%、73%、7%和 20.8%。
