Laboratorio de Microbiología, Facultad de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071 A Coruña, Spain.
Laboratorio de Microbiología, Facultad de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071 A Coruña, Spain.
Sci Total Environ. 2021 May 1;767:144363. doi: 10.1016/j.scitotenv.2020.144363. Epub 2020 Dec 29.
Standardised microalgal toxicity assays are usually carried out with single-species cultures; however, multispecies bioassays are more faithful to environmental conditions. The aim of this study was to assess the toxicity of the emerging pollutant bisphenol A (BPA) in single- and multispecies bioassays with three marine microalgae (Tetraselmis suecica, Phaeodactylum tricornutum and Nannochloropsis gaditana) using an equivalent initial cell biovolume of each species. After conducting preliminary growth experiments on these microalgae, a BPA concentration of 1 mg L was chosen to study the effects of this pollutant in uni- and multialgal cultures. Growth, cell viability, oxidative stress, and inherent cell properties (size, complexity and autofluorescence) were determined by flow cytometry after 24 h of exposure. Results showed that P. tricornutum was the most affected species in all analysed biomarkers in both single- and multispecies bioassays. However, in N. gaditana cultures no significant differences were observed in any of the parameters and conditions tested, indicating that this species was the least sensitive to BPA. Between the uni- and multialgal tests, significant differences were observed in controls and BPA treated cultures of P. tricornutum and T. suecica. Nevertheless, it seemed that the cells of these marine microalgae suffered a similar pattern of alterations in both toxicity tests.
标准化的微藻毒性测定通常使用单一物种培养物进行；然而，多物种生物测定更能反映环境条件。本研究的目的是评估新兴污染物双酚 A (BPA) 在三种海洋微藻（Tetraselmis suecica、Phaeodactylum tricornutum 和 Nannochloropsis gaditana）的单一和多物种生物测定中的毒性，使用每种物种的等效初始细胞生物量。在对这些微藻进行初步生长实验后，选择 1 mg L 的 BPA 浓度来研究这种污染物对单种和混种培养物的影响。暴露 24 小时后，通过流式细胞术测定生长、细胞活力、氧化应激和固有细胞特性（大小、复杂性和自发荧光）。结果表明，在所有分析的生物标志物中，P. tricornutum 在单一和多物种生物测定中都是受影响最严重的物种。然而，在 N. gaditana 培养物中，在所有测试的参数和条件下均未观察到显著差异，表明该物种对 BPA 的敏感性最低。在单种和多物种试验之间，在对照和 BPA 处理的 P. tricornutum 和 T. suecica 培养物中观察到显著差异。然而，似乎这些海洋微藻的细胞在这两种毒性试验中都遭受了类似的改变模式。
