Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787, Giza, Egypt.
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt; Department of Pharmaceutics, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt.
Biomed Pharmacother. 2021 Mar;135:111225. doi: 10.1016/j.biopha.2021.111225. Epub 2021 Feb 1.
Many researches have been undergone to hasten the natural wound healing process. In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated. All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium. H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity. H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid. It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios. Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients. Morphological characteristics were evaluated using scanning electron microscopy. H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE. Medicated formulations were assessed in rat model of excision wound model. The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
许多研究都致力于加速自然伤口愈合过程。在这项研究中，用石油醚、甲醇提取了几种芙蓉属植物（叶），并分离了它们的粘液。使用水溶性噻唑蓝评估了所有测试的物种提取物的存活率。选择芙蓉作为一种新的药物传递系统的组成部分，并评估其伤口愈合活性。芙蓉的石油醚提取物（PEE）含有高比例的棕榈酸和油酸，而其粘液则含有高浓度的氨基葡萄糖和半乳糖醛酸。选择它来配方，并使用壳聚糖以不同的比例制成三种不同的复合海绵，并进行药物评估。傅里叶变换红外光谱研究了所用海绵成分之间的化学相互作用。使用扫描电子显微镜评估形态特征。芙蓉粘液：壳聚糖（1:5）的复合海绵负载有三种不同浓度的 PEE。将药物配方在大鼠切除伤口模型中进行评估。临床加速、组织病理学检查以及相关炎症参数（肿瘤坏死因子）的调节，加上血管内皮生长因子的调节，清楚地证明了伤口愈合能力，这表明这是一种有前途的有价值的候选药物，可通过单次剂量制备来支持切除伤口的管理。
