Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Department of Food and Nutrition, Kookmin University, Seoul 02707, Republic of Korea.
Food Chem. 2021 Jun 1;346:128883. doi: 10.1016/j.foodchem.2020.128883. Epub 2020 Dec 24.
Bacteriophages (phages) have been extensively utilized as antibacterial agents in the food industry because of their host-specificity. However, their application in polymer films has been limited because of the lack of a strong attachment method for phage to the surface. We developed an antibacterial film by covalently immobilizing Escherichia coli (E. coli)-specific phage T4 on a polycaprolactone (PCL) film. The chemical bond formation was confirmed by XPS analysis, and the covalent attachment of phage T4 effectively inhibited E. coli growth even after external stimulation of the film by sonication. When applied as a packaging film for raw beef inoculated with E. coli O157:H7, the chemically functionalized PCL film showed approximately 30-fold higher bacterial inhibitory effects than the film with physically adsorbed phage T4. These results indicate the promising application potential of chemically functionalized PCL film with phage T4 as an antibacterial food packaging material against the foodborne pathogen E. coli.
噬菌体（phages）由于其宿主特异性，已被广泛应用于食品工业中的抗菌剂。然而，由于缺乏将噬菌体强烈附着在表面的方法，其在聚合物薄膜中的应用受到限制。我们通过将大肠杆菌（E. coli）特异性噬菌体 T4共价固定在聚己内酯（PCL）薄膜上来开发抗菌薄膜。XPS 分析证实了化学键的形成，并且即使在通过超声处理对外刺激薄膜后，噬菌体 T4 的共价附着仍能有效抑制大肠杆菌的生长。当将其用作接种有大肠杆菌 O157：H7 的生牛肉的包装膜时，化学功能化的 PCL 膜显示出比物理吸附噬菌体 T4 的膜高约 30 倍的细菌抑制效果。这些结果表明，用噬菌体 T4 化学功能化的 PCL 薄膜作为抗食源性病原体大肠杆菌的抗菌食品包装材料具有广阔的应用前景。
