Lee David J, Fontaine Arjun, Meng Xianzhong, Park Daewon
Department of Bioengineering, University of Colorado Denver Anschutz Medical Campus, 12800 East 19th Avenue, Aurora, Colorado 80045, United States.
Department of Surgery, University of Colorado Denver Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, Colorado 80045, United States.
ACS Biomater Sci Eng. 2016 Aug 8;2(8):1403-1410. doi: 10.1021/acsbiomaterials.6b00344. Epub 2016 Jul 15.
Although the gold standard for the surgical treatment of peripheral nerve injury, the autograft is associated with many drawbacks, including a second surgical procedure, donor site morbidity, mismatch of donor nerve size, and limited donor nerve length. As an alternative to the autograft, nerve guidance conduits may be used to promote neuronal growth and guide axonal extension after nerve injury. Using a blend of RGD-conjugated polyurea and polycaprolactone, a nerve guidance conduit was designed consisting of intraluminal microchannels with aligned nanofibers. A 10 mm sciatic nerve transection rat model was used to evaluate the efficacy of the conduit up to 8 weeks after nerve transection and conduit implantation. Restoration of electrophysiological activity from the nerve guidance conduit was significantly improved compared to the autograft. Functional and histological assessments indicated that the nerve guidance conduit is comparable to autograft in functional recovery and target muscle reinnervation, respectively.
尽管自体移植是周围神经损伤外科治疗的金标准，但它存在许多缺点，包括需要二次手术、供体部位发病、供体神经大小不匹配以及供体神经长度有限。作为自体移植的替代方法，神经引导导管可用于促进神经损伤后神经元生长并引导轴突延伸。使用RGD共轭聚脲和聚己内酯的混合物，设计了一种神经引导导管，其由具有排列纳米纤维的管腔内微通道组成。使用10毫米坐骨神经横断大鼠模型评估神经横断和导管植入后长达8周的导管疗效。与自体移植相比，神经引导导管的电生理活性恢复得到显著改善。功能和组织学评估表明，神经引导导管在功能恢复和靶肌肉再支配方面分别与自体移植相当。
