Yang Jie, Hsu Chia-Chen, Cao Ting-Ting, Ye Hua, Chen Jing, Li Yun-Qing
Institute of Medical Research, Northwestern Polytechnical University, Xi'an, Shaanxi Province, China.
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK.
Neural Regen Res. 2023 Mar;18(3):657-663. doi: 10.4103/1673-5374.350212.
A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro, suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regeneration. However, in vivo experiments have not been conducted. In this study, we transplanted a hyaluronic acid granular hydrogel nerve guidance conduit to repair a 10-mm long sciatic nerve gap. The Basso, Beattie, and Bresnahan locomotor rating scale, sciatic nerve compound muscle action potential recording, Fluoro-Gold retrograde tracing, growth related protein 43/S100 immunofluorescence staining, transmission electron microscopy, gastrocnemius muscle dry/wet weight ratio, and Masson's trichrome staining results showed that the nerve guidance conduit exhibited similar regeneration of sciatic nerve axons and myelin sheath, and recovery of the electrophysiological function and motor function as autologous nerve transplantation. The conduit results were superior to those of a bulk hydrogel or silicone tube transplant. These findings suggest that tissue-engineered nerve conduits containing hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of the injured sciatic nerve. The nerve conduits have the potential as a material for repairing peripheral nerve defects.
透明质酸颗粒水凝胶在体外可促进神经元和星形胶质细胞集落形成以及轴突延伸，这表明该水凝胶能够模拟细胞外基质结构以促进神经再生。然而，尚未进行体内实验。在本研究中，我们移植了透明质酸颗粒水凝胶神经导管以修复10毫米长的坐骨神经缺损。Basso、Beattie和Bresnahan运动评分量表、坐骨神经复合肌肉动作电位记录、荧光金逆行示踪、生长相关蛋白43/S100免疫荧光染色、透射电子显微镜检查、腓肠肌干/湿重比以及Masson三色染色结果表明，该神经导管在坐骨神经轴突和髓鞘再生、电生理功能和运动功能恢复方面表现出与自体神经移植相似的效果。导管的效果优于块状水凝胶或硅胶管移植。这些发现表明，含有透明质酸颗粒水凝胶的组织工程神经导管可有效促进受损坐骨神经的形态和功能恢复。这些神经导管有潜力作为修复周围神经缺损的材料。
