Xu Xiongcheng, Lu Yanjin, Yang Xue, Du Zhibin, Zhou Ling, Li Shuman, Chen Chao, Luo Kai, Lin Jinxin
Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Zhong Road, Fuzhou 350002, China.
Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, 155 West Yangqiao Road, Fuzhou 350002, China.
ACS Biomater Sci Eng. 2018 Sep 10;4(9):3364-3373. doi: 10.1021/acsbiomaterials.8b00736. Epub 2018 Aug 17.
Copper has been reported to promote bone regeneration by increasing osteogenesis and decreasing inflammation and osteoclastogenesis. However, information on the effects of copper on osteoporotic cells involved in bone regeneration is scarce in the literature. In the current study, Ti6Al4 V-6 wt %Cu (Ti6Al4 V-Cu) was fabricated by selective laser melting (SLM) technology, and the effects of copper on the behaviors of osteoporotic and nonosteoporotic macrophages, osteoclasts, and osteoblasts were evaluated by comparison with Ti6Al4 V. Our results showed that Ti6Al4 V-Cu inhibited the activation, viability, and pro-inflammatory cytokine secretion of osteoporotic macrophages and decreased osteoclast formation and down-regulated osteoclast differentiation-related genes and proteins of osteoporotic osteoclasts. Furthermore, the bone extracellular matrix formation of osteoporotic osteoblasts was up-regulated by Ti6Al4 V-Cu. In conclusion, SLM-fabricated Ti6Al4 V-Cu exhibited excellent anti-inflammation and antiosteoclast capability, optimized extracellular matrix formation, and holds great potential for bone regeneration in osteoporotic patients.
据报道，铜可通过促进成骨作用、减轻炎症反应和抑制破骨细胞生成来促进骨再生。然而，文献中关于铜对参与骨再生的骨质疏松细胞影响的信息却很少。在本研究中，采用选择性激光熔化（SLM）技术制备了Ti6Al4 V-6 wt %Cu（Ti6Al4 V-Cu），并通过与Ti6Al4 V对比，评估了铜对骨质疏松和非骨质疏松巨噬细胞、破骨细胞和成骨细胞行为的影响。我们的结果表明，Ti6Al4 V-Cu抑制了骨质疏松巨噬细胞的活化、活力和促炎细胞因子分泌，减少了破骨细胞形成，并下调了骨质疏松破骨细胞中与破骨细胞分化相关的基因和蛋白质。此外，Ti6Al4 V-Cu上调了骨质疏松成骨细胞的骨细胞外基质形成。总之，SLM制备的Ti6Al4 V-Cu具有优异的抗炎和抗破骨能力，优化了细胞外基质形成，在骨质疏松患者的骨再生方面具有巨大潜力。
